The fructosamine (total glycated serum proteins) level was determined according to the manufacturer's instruction (BSBE, China). After the mice were fasted for 4 h, FBG was detected using Accu-Chek blood glucose meters (Roche, USA). Glucose tolerance test (GTT) was performed in the animals after fasting for 4 h followed by intraperitoneal injection with glucose solution (2.0 g/kg). The blood glucose level was determined at 0, 30, 60, and 120 min after glucose loading using Accu-Chek blood glucose meters (Roche, USA).
Accu chek blood glucose meter
Manufactured by Roche
Sourced in China, United States, Switzerland
The Accu-Chek blood glucose meter is a portable device used to measure the concentration of glucose in a person's blood. It provides accurate and reliable blood glucose measurements, which are essential for managing conditions such as diabetes.
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Lab products found in correlation
Streptozotocin (stz), by Merck Group (3 mentions)
Accu chek active glucose strips, by Roche (1 mentions)
Pai 1 total mouse elisa kit, by Abcam (1 mentions)
7150 automatic biochemical analyzer, by Hitachi (1 mentions)
Sds page electrophoresis, by Bio-Rad (1 mentions)
1230 transmission electron microscope, by JEOL (1 mentions)
Goat anti rabbit antibodies, by Boster Bio (1 mentions)
Gel doc xr gel imager, by Bio-Rad (1 mentions)
Microplate reader, by Bio-Rad (1 mentions)
Nefa c, by Fujifilm (1 mentions)
Triglyceride e, by Fujifilm (1 mentions)
Cholesterol e, by Fujifilm (1 mentions)
Calcium e, by Fujifilm (1 mentions)
Relative assay kit, by Solarbio (1 mentions)
Insulin elisa kit, by Cloud-Clone (1 mentions)
Glucose assay kit, by Merck Group (1 mentions)
P nitrophenyl α d glucopyranoside, by Merck Group (1 mentions)
Silica gel 60 f254 glass plates, by Merck Group (1 mentions)
α glucosidase, by Merck Group (1 mentions)
Acarbose, by Merck Group (1 mentions)
22 protocols using accu chek blood glucose meter
1
Fructosamine and Glucose Tolerance Test
2
Induction of Diabetic Nephropathy in Mice
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After 10 days of normal feeding, the animals were fasted for 12 h prior to the intraperitoneal injection of STZ (150 mg/kg) dissolved in citrate buffer (pH 4.5). The injection was administered once in all the groups of mice with the exception of the normal control group. After 72 h, blood samples were collected from the tails, to perform a blood sugar test (ACCU-CHEK blood glucose meter; Roche Diagnostics, GmbH, Mannheim, Germany) and 24-h microalbuminuria test. The induction of diabetes was considered successful when blood sugar was >16.17 mmol/l. The same amount of sterile citrate buffer was injected intraperitoneally in mice of the control group. The mice were then fed with a high-fat diet for one month, and the blood sugar test and 24-h microalbuminuria test were repeated. The establishment of the mouse model of diabetic nephropathy was confirmed with the blood sugar as >16.17 mmol/l and the quantification of 24-h urinary albumin increased to >150%.
3
Measuring Glucose Metabolism in Mice
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Blood glucose levels were measured every 2 weeks from a drop of blood by tail incision using Accu-Chek blood glucose meter and Accu-Chek Active glucose strips (ROCHE, Switzerland). Whereas, glycated hemoglobin (HbA1c) was assessed using the BIOHRMES HbA1c Analyzer (BIOHERMES, USA) at the 8th week following the first injection, and the 6th week following the second injection. Besides, the intraperitoneal glucose tolerance test (IPGTT) was performed using the Accu-Check blood glucose meter. Briefly, after six hours of fasting, mice body weights and fasting blood glucose levels were recorded (t = 0 min). Mice were then injected with 20% glucose. The volume of the glucose injection was calculated according to the formula: volume = 10 × lean body mass (g)41 . Blood glucose levels were then measured at 15, 30, 60 and 120 min post-injection.
4
Rat Blood Glucose and Insulin Measurement
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When the blood was obtained from each rat, it was quickly measured
using an ACCU-CHEK blood glucose meter (Roche Diagnostics Ltd. Company,
Shanghai, China). Insulin levels were measured using an insulin ELISA
kit (Jiangsu Jiancheng Company, Nanjing, China).
using an ACCU-CHEK blood glucose meter (Roche Diagnostics Ltd. Company,
Shanghai, China). Insulin levels were measured using an insulin ELISA
kit (Jiangsu Jiancheng Company, Nanjing, China).
5
Metabolic Profiling in Diabetic Mice
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At 5 days after STZ injection and during week 15 of the HFD, mice were fasted overnight (12 h) and blood samples were collected by cutting the tip of the tail. Fasting serum glucose levels were measured using an Accu-Chek blood glucose meter (Roche Diagnostics, Castle Hill, Australia). The levels of serum cholesterol, triglycerides, and blood urea nitrogen (BUN) were determined in serum with an Idexx VetTest Biochemical Analyzer (Westbrook, ME, USA). The serum PAI-1 levels, which include active, inactive, and latent forms of PAI-1, were assessed using a PAI-1 Total Mouse ELISA kit (Abcam) according to the manufacturer’s instructions.
6
Diabetic Kidney Disease Evaluation
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CS was purchased from Jimin Pharmaceutical Co., Ltd., (Jiangxi, China), TWP was puchased from Fudan Fuhua Pharmaceutical Co., Ltd., (Shanghai, China), streptozotocin was obtained from Sigma (St. Louis, MO, USA), rat urine albumin EIA assay kit was obtained from R&D Systems (Minneapolis, MN, USA), rabbit anti-rat nephrin antibodies, rabbit anti-rat podocin antibodies and goat anti-rabbit antibodies were purchased from Boster (Wuhan, China), Accu-chek Blood glucose meter was obtained from Roche Diagnostics GmbH (Mannheim, Germany). The 7150 automatic biochemical analyzer was purchased from Hitachi (Tokyo, Japan), the JEOL-1230 transmission electron microscope was obtained from JEOL (Tokyo, Japan), Vanox multifunctional microscope was purchased from Olympus (Tokyo, Japan) and SDS-PAGE electrophoresis was obtained from Bio-Rad (Hercules, CA, USA).
7
Rat Model of Type 2 Diabetes
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Diabetes mellitus (DM) was induced by feeding the rats a high-fat diet (HFD) (total energy 25.07 kJ/g including fat 60%, protein 20%, and carbohydrate 20%) for 4 weeks. After that, the rats were fasted overnight. STZ (Sigma, St. Louis, MO, USA) was freshly prepared in a 0.05 M citrate buffer (pH 4.5), and a single intraperitoneal (i.p.) injection of 35 mg kg−1 was used to induce T2DM in each rat. The blood glucose level was monitored every 3 days using an Accu-Chek blood glucose meter (Roche Diagnostics, Basel, Switzerland). Stable hyperglycemia was established in the rats (denoted by blood glucose levels ≥ 200 mg/dl (15 mM)) seven days after the STZ injection.
Fourteen rats acted as the control where they were injected with citrate buffer. After that, STZ at 35 mg kg−1 was injected intraperitoneally [18 (link)]. Rats with plasma glucose levels of 150–280 mg/dl were selected for the following experiment.
Fourteen rats acted as the control where they were injected with citrate buffer. After that, STZ at 35 mg kg−1 was injected intraperitoneally [18 (link)]. Rats with plasma glucose levels of 150–280 mg/dl were selected for the following experiment.
8
Oral Glucose Tolerance Test in Rats
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The oral glucose tolerance test (OGTT) was performed at the Thursday in week 8 (day 53). Followed a 14-h fasting, all rats were orally administrated with 50% glucose solution (1.5 g/kg), and the tail vein blood were collected at 0, 30, 60, 90, 120 min after administration. The blood glucose was assayed by an ACCU-CHEK blood glucose meter (Roche Diagnostics Ltd. Company, Shanghai, China).
9
Graft Function Evaluation in Intestinal Transplantation
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To evaluate graft absorptive and barrier function, a solution containing glucose (2 g/kg) and ovalbumin (OVA) (150 μg/dose) diluted in 1.5-mL saline was administered by proximal ostomy 5 and 10 days after ITX. Glycemia was measured using an Accu-Chek blood glucose meter (Roche) just before and 15, 30, 60, 90, 120, and 180 minutes after glucose administration. Serum levels of OVA were measured by competitive ELISA 30 and 90 minutes after administration as previously described.11 For both measurements, blood samples were obtained from the receptor tail vein. Also, graft contractile function by isometric graft force measurements was analyzed at 4 and 10 POD (Materials and Methods, SDC, http://links.lww.com/TXD/A52 ).
10
Fasting Blood Glucose Monitoring in Diabetic Rats
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After the start of treatment, fasting blood glucose of the rats in the control group, STZ group, STZ+OLE group, and STZ+SITA group was measured every week. All rats fasted for 12 h before the test but were given free access to water. Subsequently, the rats were fixed, and 1/4 of the end of the tail was disinfected with alcohol cotton balls and was then punctured with a blood lancet to take an appropriate amount of blood. The blood was dropped onto the test area of the blood glucose test strip, and the strip was then immediately read by the ACCU-CHEK blood glucose meter (Roche Diagnostics, Shanghai, China).
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