Antibodies were used as follows: Krt14 (generous gift of Dr. Segre, National Human Genome Research Institute, MD, USA, 1:20,000); H3 (abcam, ab1791); H3K27me3 (Millipore, 07-449, 1:300); Krt18 (abcam, ab668, 1:100); Krt20 (Dako, M7019, 1:70); Sox2 (Stemgent, 09-0024, 1:150); Isl1 (abcam, ab109517, 1:250); NF200 (abcam, ab8135, 1:1000); Krt6 (generous gift of Dr. Fuchs, The Rockefeller University, NY, USA, 1:250); Lhx2 (generous gift of Dr. Fuchs, 1:5000); Sox9 (generous gift of Dr. Fuchs, 1:1000); BrdU (abcam, ab6326, 1:250; abcam, ab1893, 1:250); AcCasp3 (R&D, AF835, 1:250); Krt10 (Covance, PRB-159P, 1:500); Loricrin (Covance, PRB-145P, 1:250); Filaggrin (generous gift of Dr. Segre, 1:500); Integrin β4/ CD104 (BD Biosciences, 553745, 1:500); Ki67 (Novocastra, NCL-L-Ki67-MM1, 1:250); AE13 (Abcam, ab16113, 1:100); E-Cadherin (Invitrogen, 131900, 1/2000); p19/Arf (Abcam, Ab80, 1/200). For IF, secondary Abs coupled to FITC, Alexa488, 549, 649, RRX, or Cy5 were from Jackson Laboratories (1:1000). For FACS: anti-mEphrin-B1 (BAF473 R&D), FITC-Streptavidin (554061 BD), Ep-CAM-APC (118214, BioLegend), Ly-A6 Sca1-Cy5.5 (45-5981-82, eBioscience), CD49f-α6 integrin PE (11-0495-82, eBioscience). For Western blot, TrueBlot Anti-Rabbit IgG HRP (Rockland, 18-8816-33, 1:10,000) was used as a secondary Ab.
Ncl l ki67 mm1
Manufactured by Abcam
Sourced in United States
NCL-L-Ki67-MM1 is a monoclonal antibody that recognizes the Ki-67 protein, a cellular marker for proliferation. It is used in immunohistochemical (IHC) and immunocytochemical (ICC) applications to detect the presence and expression level of Ki-67 in cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Epcam apc, by BioLegend (1 mentions)
Krt20, by Agilent Technologies (1 mentions)
Streptavidin fitc, by BD (1 mentions)
Fluorescein isothiocyanate (fitc), by Jackson ImmunoResearch (1 mentions)
E cadherin, by Thermo Fisher Scientific (1 mentions)
P19arf, by Abcam (1 mentions)
H3k27me3, by Merck Group (1 mentions)
Alexa 488, by Jackson ImmunoResearch (1 mentions)
Ab87099, by Abcam (1 mentions)
2 protocols using ncl l ki67 mm1
1
Immunohistochemical Profiling of Skin Samples
2
Ovarian Cancer Tissue Microarray Analysis
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Tissue microarrays (TMA) containing 70 (OV701) and 99 (OV991) ovarian cancer samples were purchased from US Biomax. These materials were commercially available anonymized and de‐identified. Since the tissues were commercially purchased, the study has been exempted from requiring ethical approval. Paraffin‐embedded tissue slides were baked overnight, then de‐waxed, and rehydrated according to standard protocols. Tissue sections were then subjected to antigen retrieval in TRIS‐EDTA at 95°C for 20 min (pH = 9). The solution is then cooled for an additional 20 min on the benchtop prior to buffer rinse. Tissues were then incubated overnight with rabbit anti‐glutaminase (Abcam, Cat# ab156876), mouse anti‐GS (Sigma MAB302), mouse anti‐Ki67 (Novacastra, NCL‐L‐Ki67‐MM1), and rabbit anti‐CD163 (Abcam, ab87099) in a humid chamber in the dark at room temperature. Appropriate secondary antibodies Alexa 488 and 594‐conjugated (Invitrogen) were used. The slides were then mounted using nucleus‐specific DAPI counterstain and coverslipped. Slides were then imaged using fluorescence microscopy on a Nikon Eclipse 80i‐ViCO.
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