Rat anti mouse f4 80 monoclonal antibody
The Rat anti-mouse F4/80 monoclonal antibody is a laboratory reagent used for the identification and characterization of mouse macrophages. It recognizes the F4/80 antigen, which is a glycoprotein expressed on the surface of mouse macrophages. This antibody can be used in various immunological techniques, such as flow cytometry, immunohistochemistry, and immunoprecipitation, to detect and study mouse macrophages.
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7 protocols using rat anti mouse f4 80 monoclonal antibody
Macrophage Visualization in Sciatic Nerve
Immunohistochemical Analysis of Tumor Angiogenesis and Macrophages
Immunohistochemical Analysis of Kidney Markers
Alternatively, frozen sections were incubated in the widely used markers of murine macrophage populations, rat anti-mouse F4/80 monoclonal antibody (dilution 1:100, ABD Serotec, USA) or rat anti-mouse CD68 antibody (dilution 1:100, ABD Serotec) for one hour. Thereafter, they were incubated with a secondary HRP labelled goat anti-rat antibody for 30 min (ABD Serotec, dilution 1:200).
Antigen-antibody reactions were visualized with 3.3diaminobenzidine tetrahydrochloride (Dako) and counterstained. The tissue specimens were examined by light microscopy using the Olympus photomicroscope. For fibronectin, collagen IV, 8-OHdg, CD68, and F4/80, six consecutive non-overlapping fields from each section of renal cortex were photographed under high magnification. Stained areas were quantified using Image J software (NIH, UK).
Histological Analysis of Spinal Cord in EAE Mice
Histological Analysis of Liver Fibrosis
Mouse Macrophage F4/80 Immunohistochemistry
Mouse Model of Inflammation
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