Spss statistics software v 22

Statistical analyses were performed using SPSS Statistics software V. 22.0 (IBM, Armonk, NY, USA). We obtained means and standard deviations. In the osteoclastogenesis study, group means were compared using the Kruskal–Wallis test. The Wilcoxon’s signed-rank test was used to determine significant differences between fibrinogen and citrullinated fibrinogen in osteoclast gene expression.

Statistical analysis was performed using Mann–Whitney U tests, using SPSS Statistics software V. 22.0 (IBM, Armonk, NY, USA). Data are presented as the mean ± standard error of the mean (SEM). p-values less than 0.05 were considered statistically significant.

Non-parametrical statistical analyses with exact significance values were used for all group comparisons. Comparisons between the genotype groups were performed using the independent-samples Mann-Whitney U test. Differences between the various age groups were evaluated with the independent-samples Kruskal–Wallis test. Post hoc analysis between specific age groups was performed using the independent-samples Mann–Whitney U test with a Bonferroni correction for multiple comparisons. All statistical tests were performed using SPSS statistics software v22.0 (IBM, Amrock, NY, USA). All graphs were created using Graphpad Prism v5.03 (Graphpad, San Diego, CA, USA).

The defined event was death from any cause. PFS was defined as the time between date of first treatment and date of tumor progression and OS was defined as time from diagnosis of locally advanced or metastatic disease until date of death; or if event was not found, censored at date of last observation.
Kaplan-Meier plots were used for PFS and OS analysis, and the log-rank test was used to compare curves separated according to expression of p53. Cox proportional regression was performed for the estimation of hazard ratios (HRs) and confidence intervals (CIs).
Spearman correlation was used to assess the correlation of p53 protein expression to clinicopathological variables. For the statistical analyses all variables were dichotomized: p53 IR vs. non-IR, age ≤60 years vs. >60 years, Ki67 ≥55% vs. ≤55% LDH normal vs. high and performance status ECOG 0+1 vs. 2+3.
All statistical analyses were performed using IBM SPSS statistics software (v22, USA).