Bs010

The MC simulation modeled our current 2D-DWP-based sSMLM system as previously reported.¹³ (link) We used two excitation lasers (532 nm, Exlsrone-532-200, Spectra-Physics; and 647 nm, 2RU-VFL-P-2000-647-B1R, MPB Communications) and an inverted microscope body (Ti2-E, Nikon) equipped with a 100× total-internal reflection fluorescence (TIRF) objective (CFI Apochromat TIRF 100XC Oil, Nikon). Figure 1(a) shows the photon flow in the detection path. The emitted photons from the fluorescent sample pass through a DWP module, consisting of a BS prism (BS010 for R50:T50; and BS049 for R30:T70, Thorlabs), a right-angle prism (RAP) (84-514, Edmund Optics), and a custom-made DWP.¹³ (link) The BS divides the photons into a spatial imaging (zeroth order) path and a spectral imaging (first order) path. The RAP then reflects photons in the spatial imaging path, and the custom-made DWP disperses the photons in the spectral imaging path. Finally, an electron-multiplying charge-coupled device (EMCCD) (iXon Ultra 897, Oxford Instruments) simultaneously detects all of the photons in the zeroth order and first order paths for further processing.