Antibodies used in this study were as follows: rabbit polyclonal anti-DDX6 (Bethyl Laboratories), rabbit polyclonal anti-Pat1b (Bethyl Laboratories), rabbit polyclonal anti-DCP2 (Bethyl Laboratories), mouse monoclonal anti-EDC3 (Abcam), rabbit polyclonal anti-GW182 (Bethyl Laboratories), mouse monoclonal anti-eIF4E (BD Transduction Laboratories), rabbit polyclonal anti-PABP (Abcam), rabbit monoclonal anti-Ago2 (Cell Signaling), mouse monoclonal anti-myc (BioShop Canada Inc.), mouse monoclonal anti-β-actin (Sigma). Rabbit polyclonal antibodies against CNOT1 and CNOT7 were kindly provided by T. Yamamoto.
Anti ddx6
Manufactured by Fortis Life Sciences
Sourced in Canada
Anti-DDX6 is a laboratory reagent that targets the DDX6 protein, which is a member of the DEAD-box RNA helicase family. It is used in research applications to study the role and function of the DDX6 protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti pabp, by Abcam (1 mentions)
Rabbit monoclonal anti ago2, by Cell Signaling Technology (1 mentions)
Anti gw182, by Fortis Life Sciences (1 mentions)
Mouse monoclonal anti β actin, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Nacalai Tesque (1 mentions)
Gtx632269, by GeneTex (1 mentions)
Protein g sepharose, by GE Healthcare (1 mentions)
Anti gigyf2, by Santa Cruz Biotechnology (1 mentions)
Anti 14 3 3, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Mouse monoclonal anti flag m2, by Merck Group (1 mentions)
3 protocols using anti ddx6
1
Antibody Validation for mRNA Decay Factors
2
SARS-CoV-2 Nucleocapsid Protein Interactome
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were lysed in buffer containing 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 4 mM EDTA, 0.1% NP-40, 10 mM NaF, protease inhibitor cocktail (Nacalai Tesque, Kyoto, Japan). Lysates were precleared with 30 μL of protein-G-Sepharose (GE Healthcare Bio-Sciences). Precleared supernatants were incubated with 5 μg of anti-SARS-CoV-2 nucleocapsid antibody mixture (GTX135357 and GTX632269 [6H3]; GeneTex) or anti-DDX21 (A300-627A; Bethyl Lab), anti-DDX6 (A300-460A; Bethyl Lab), or anti-MOV10 (A301-571A; Bethyl Lab) antibody at 4°C for 1 h. Following absorption of the precipitates on 30 μL of protein G-Sepharose resin for 1 h, the resin was washed four times with 700 μL of lysis buffer. Proteins were eluted by boiling the resin for 5 min in 2× Laemmli sample buffer. The proteins were then subjected to SDS-PAGE, followed by immunoblot analysis using anti-SARS-CoV-2 nucleocapsid (GTX632269 [6H3]), anti-DDX21, anti-DDX6, or anti-MOV10 antibody.
3
Antibody Generation and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rabbit polyclonal anti-pS316-TTP antibody was generated with the synthesized oligo-peptide PRRLPIFNRI(p)SVSE (Pocono Rabbit Farm & Laboratory, USA). The antiserum was purified by affinity chromatography by first collecting antibody binding to a PRRLPIFNRI(p)SVSE-peptide column, and, after elution, collecting the flow-through from a subsequent unphosphorylated PRRLPIFNRISVSE-peptide column. Immunoblots were probed with the following antibodies at the indicated concentrations: rabbit polyclonal anti-FLAG (Millipore Sigma, F7425; 1:1,000), mouse monoclonal anti-FLAG M2 (Millipore Sigma, F1804; 1:1,000), rabbit polyclonal anti-DDX6 (Bethyl, A300-461A; 1:1,000), rabbit polyclonal anti-GIGYF2 (Santa Cruz Biotechnology, sc-134708; 1:50), mouse monoclonal anti-14-3-3 (Santa Cruz Biotechnology, sc-1657; 1:1,000), rabbit polyclonal anti-TTP (Sigma-Aldrich, T5327; 1:500), rabbit polyclonal anti-CNOT1 (Proteintech, 14276-1-AP; 1:200), rabbit polyclonal anti-CNOT9 (Proteintech, 22503-1-AP, 1:500), mouse monoclonal anti-GAPDH (Cell Signaling Technology, 97166, 1:1,000), rabbit polyclonal anti-EDC4 ((42 (link)); 1:200) rabbit polyclonal anti-pSer178-TTP ((11) a generous gift from Dr. Georg Stoecklin, Heidelberg University; 1:500).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!