Erastin c30h31cln4o4

Indicated HeLa cells were subcutaneously injected into the dorsal flanks right of the midline in SCID mice (weight ~20g). At day seven, mice were injected with Erastin (20 mg/kg/ i.v., twice daily every other day) with or without KRIBB3 (50 mg/kg/ i.p., once daily every other day) for two weeks. The Erastin (C₃₀H₃₁ClN₄O₄) was bought from Selleck Chemicals. Erastin was dissolved in vehicle (2% DMSO and 98% phosphate buffered saline) and prepared by Ultrasonic Cleaner (Fisher Scientific). A final volume of 300 ul Erastin was applied through the tail vein. The half-life and area under curve (0-t) of Erastin are 27 min and 173 µg.min/ml, respectively. The Rodent Tail Vein Catheter (Braintree Scientific, MTV#1) were used to perform injection. This system allows easier access for both timed injections and repeated bolus administration. Tumors were measured once a week. The volumes were calculated using the following formula: volume (mm³)=length×width²×π/6. This study was approved by the Third Affiliated Hospital of Guangzhou Medical University and the University of Pittsburgh Institutional Animal Care and Use Committees and performed in accordance with the Association for Assessment and Accreditation of Laboratory Animal Care guidelines (http://www.aaalac.org).