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4 protocols using petroleum ether

1

Fatty Acid Extraction Protocol

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Sodium chloride, hydrogen chloride, diethyl ether, and petroleum ether were purchased from Daejung Chemicals and Metals Co., Ltd. (Shiheung, Korea) . Triundecanoin (C11:0) , which was used as the internal standard for FA analysis, was purchased from Nu-Chek Prep, Inc. (Elysian, MN, USA) . Analytical grade isooctane and anhydrous sodium sulfate were obtained from Fisher Scientific Korea (Seoul, Korea) and Junsei Chemical Co., Ltd. (Tokyo, Japan) , respectively. Analytical standards for FA methyl esters (Supelco 37 component FAME mix) , pyrogallol, and BF 3 -methanol (14%, w/w) were obtained from Sigma-Aldrich (Seoul, Korea) .
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2

Fatty Acid Methyl Esters Analysis Protocol

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Standard of fatty acid methyl esters (37 components FAME mix), BF3-methanol (10%, w/w), pyrogallol and α-tocopherol (≥96%, HPLC) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). C11:0 triundecanoin as triglyceride internal standard was obtained from Nu-Chek Prep, Inc. (Elysian, MN, USA). β-carotene (≥95%, HPLC) was provided by Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Anhydrous sodium sulfate and 2,6-Di-tert-butyl-4-methylphenol (BHT) were from Junsei Chemical Co., Ltd. (Tokyo, Japan). KOH, NaCl, diethyl ether, hexane, ethyl acetate, ethanol, chloroform, and petroleum ether were analytical grade and acquired from Daejung Chemicals & Metals Co., Ltd. (Shiheung, South Korea). HPLC grade of chloroform, hexane, methanol, and iso-propanol were obtained from Fisher scientific Korea Ltd. (Seoul, Republic of Korea).
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3

Synthesis of Organic Compounds

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Toluene (99.8%), methyl alcohol (99.9%), and nitric acid (68.0–70.0%) were purchased from Samchun. Tetrahydrofuran (THF) was obtained from Duksan. Hydrochloric acid (36.5–38.0%) was ordered from J.T.Baker. Silk fibroin (5% solution) was purchased from Advanced BioMatrix. Sodium nitrite, 6-bromo-1-hexene, chloroplatinic acid hexahydrate (H2PtCl6 ∙ 6H2O), decanoic acid, hydrazine monohydrate, silver acetate, dodecylamine (DDA), lead(II) bromide, methylammonium bromide (MABr), 4-methoxyaniline, sodium hydride, and tetrabutylammonium borohydride (TBAB) were obtained from Sigma-Aldrich. AZ-5214E was used as photoresist (PR). Sodium hydroxide, phenol, and petroleum ether were ordered from Daejung. N,N-dimethylformamide (DMF) was purchased from Honeywell. Silicone elastomer base and silicone elastomer curing agent (Sylgard 184) were obtained from Dow Corning.
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4

Phytochemical Analysis and Antioxidant Activity

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Oleanolic acid, hederagenin, and cholesterol were purchased from Sigma‐Aldrich. Phytolaccagenic acid was purchased from Carbosynth. Oleanolic acid and phytolaccagenic acid were dissolved in Ethyl acetate while hederagenin was dissolved in methanol. Then, each standard was prepared at a concentration of 1 mg/ml. Ammonia water, N,O‐Bis(trimethylsilyl) trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS), anhydrous pyridine, ethanol, rutin, gallic acid, folin–ciocalteu reagent, 2,2‐diphenyl‐1‐picrylhydrazyl, 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt, phosphate buffer (pH 6.6), potassium hexacyanoferrate(III), trichloroacetic acid, and iron(III) chloride were purchased from Sigma‐Aldrich. Sodium sulfate anhydrous, hydrochloric acid, sodium carbonate, sodium hydroxide, petroleum ether, glacial acetic acid, and sulfuric acid were purchased from Daejung Chemicals & Metals. Ethyl acetate and methanol were purchased from J. T Baker.
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