Gfp antibody ab290

Cells were transfected, and then harvested for the co-transfection with pcDNA3.1-MS2 and pcDNA3.1-MS2-LINC01419 for 48 h. Samples were then subjected to RIP assay with GFP antibody (ab290, 1:200 dilution; Abcam) by use of RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Bedford, MA). Finally, qRT-PCR was exploited to analyze the level of co-precipitated RNAs.

LacZ (40–1A), Ci (2A1) and cut (2B10) antibodies were obtained from the Developmental Studies Hybridoma Bank. GFP antibody (ab290) and hairy antibody (ab20165) were from Abcam.

Fragments (native promoters and ORFs) of FonAP-2α, FonAP-2β, FonAP-2μ, and the palmitoylation-defeat variants FonAP-2α^C400S and FonAP-2μ^C24S were inserted into vectors pYF11 (GFP tag) or pHZ126 (3×FLAG tag), which were then cotransformed into the WT. The strains bearing FonAP-2α-GFP plus FonAP-2β-FLAG, FonAP-2α^C400S-GFP plus FonAP-2β-FLAG, FonAP-2μ-GFP plus FonAP-2β-FLAG, FonAP-2μ-GFP^C24S plus FonAP-2β-FLAG, or a single construct were generated and confirmed by their antibiotic resistance, PCR amplification of the target constructs, and Western blotting of the proteins with anti-FLAG or anti-GFP antibodies. For co-IP assays, total proteins were extracted and incubated with GFP-Trap beads (ChromoTek, Planegg-Martinsried, Germany) as previously described (46 (link)). The eluted proteins were detected with GFP antibody (ab290, Abcam, Cambridge, UK) or FLAG antibody (Sigma-Aldrich, St. Louis, MO, USA).