Uv mini 1240 ce

Carvedilol release from the various formulations was evaluated using the dialysis method. One milliliter of nanoparticle dispersion was placed into a dialysis bag (cutoff 12 kDa) and suspended into a beaker containing 70 mL of a phosphate buffer solution (pH 7.4) on a magnetic stirrer, with a speed of 100 rpm at 37°C ± 0.5°C. At pre-determined time intervals, 1 mL samples were withdrawn from the incubation medium and analyzed for the drug content by a UV spectrophotometer (UV-mini-1240 CE-Shimadzu, Japan) at λ_max = 255 nm. The drug release tests were performed in triplicate.

The specified amount of LC formulation was centrifuged (Sigma, US) at 8000 rpm for 20 min. Then, the clear supernatant liquid was decanted. The dissolved FFB in the liquid phase was measured using spectrophotometer (UV-mini-1240 CE-Shimadzu, Japan) at λ_max = 228 nm. Then, the percent of the drug loaded into LCs was calculated according to the initial drug payload.