Minocycline hydrochloride and Tetracycline hydrochloride (Sigma-Aldrich) were resuspended in water at 4 mg/ml, before dilution in RPMI to the required concentration. Tigecycline hydrate (Sigma-Aldrich) was resuspended in DMSO at 5 mg/ml, before dilution in RPMI. Stock solutions were made fresh for each experiment. Typically for 96-well plate assays, 50 μl of stock solution or cRPMI control was added per well.
Tigecycline hydrate
Manufactured by Merck Group
Sourced in United States
Tigecycline hydrate is a laboratory compound produced by Merck Group. It is a tetracycline antibiotic that can be used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Ciprofloxacin hydrochloride monohydrate, by Fujifilm (2 mentions)
Piperacillin, by Merck Group (2 mentions)
Colistin sulfate, by Merck Group (2 mentions)
Azithromycin dihydrate, by Merck Group (2 mentions)
Amikacin disulfate salt, by Merck Group (2 mentions)
K pneumoniae and e coli strains, by American Type Culture Collection (2 mentions)
Tetracycline hydrochloride, by Merck Group (1 mentions)
Minocycline hydrochloride, by Merck Group (1 mentions)
Minocycline hcl, by Merck Group (1 mentions)
Oxytetracycline hcl, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Demeclocycline hydrochloride, by Merck Group (1 mentions)
Ctc hydrochloride, by Merck Group (1 mentions)
Tet hydrochloride, by Merck Group (1 mentions)
Tigecycline d9, by LGC (1 mentions)
Tygacil, by Pfizer (1 mentions)
Bacto agar, by BD (1 mentions)
Luria bertani broth, by BD (1 mentions)
Quercetin, by Merck Group (1 mentions)
Catechin hydrate, by Merck Group (1 mentions)
8 protocols using tigecycline hydrate
1
Antibacterial Compound Preparation and Dosing
2
Tetracycline Antibiotic Formulations and Dosing
3
Quantitative Determination of Tetracycline Analogues
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Tigecycline hydrate (CAS 220620-09-7), CTC hydrochloride (CAS 64-72-2), TET hydrochloride (CAS 64-75-5), demeclocycline hydrochloride (CAS 64-73-3) as the internal standard (IS ) for CTC, formic acid, acetonitrile, 1,2-dichloroethane, and water were purchased from Sigma-Aldrich (St. Louis, MO). Minocycline hydrochloride (CAS 13614-98-7) was supplied by Thermo Fisher Scientific (Waltham, MA). Tigecycline-d9 (CAS unlabeled) as the IS for TIG and MIN, and doxycycline-d3 hyclate (CAS unlabeled) as the IS for TET were purchased from Toronto Research Chemicals (North York, ON, Canada). For chromatographic analysis, the working solutions of each drug were prepared by diluting stock solutions (in methanol) in 0.1% formic acid in water.
Analytical standards of CTC, TET, and MIN dissolved in deionized water were also used for administration to animals during the PK experiment. Only TIG was not of an analytical standard due to the unavailability of TIG hydrochloride salt; therefore commercial product Tygacil (Pfizer, New York, NY) which contains a hydrochloric acid was used (TIG, lactose monohydrate, hydrochloric acid, sodium hydroxide).
Analytical standards of CTC, TET, and MIN dissolved in deionized water were also used for administration to animals during the PK experiment. Only TIG was not of an analytical standard due to the unavailability of TIG hydrochloride salt; therefore commercial product Tygacil (Pfizer, New York, NY) which contains a hydrochloric acid was used (TIG, lactose monohydrate, hydrochloric acid, sodium hydroxide).
4
Bacterial Growth in LB Broth and Agar
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The bacterial strains used in this study are listed in Table 1 . Bacterial growth was performed using Luria-Bertani (LB) broth (BD BioSciences, San Jose, CA, USA) with or without 1.5% of BactoTM Agar (BD BioSciences, San Jose, CA, USA) (named LB-agar) and 37 °C was set as a culture temperature unless indicated otherwise. Tigecycline hydrate and other chemicals used in this study were purchased from Sigma-Aldrich (Sigma-Aldrich, Saint Louis, MO, USA).
5
Purification and Characterization of Bioactive Compounds
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Fatty-acid-free human serum albumin (HSA, A1887), tigecycline hydrate (TGC, PZ0021), phosphate-buffered saline (PBS, P4417), catechin hydrate (CAT, 22110), and quercetin (QUE, Q4915) were purchased from Sigma–Aldrich Chemical Company (St. Louis, MO, USA) and used without further purification. Caffeine (CAF, 326356) was purchased from the Carlo Erba reagents. Diosmin (DIO, D3525) was purchased from Sigma Aldrich (Fluka). All starting materials were high-purity grade and used as purchased without further purification. The deionized water was used for preparing all solutions.
6
Antimicrobial Susceptibility Testing of Klebsiella and E. coli
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K. pneumoniae and E. coli strains were purchased from the American Type Culture Collection (Manassas, VA) or the National Collection of Type Cultures (London, United Kingdom). Cation-adjusted Mueller-Hinton broth was used for antibacterial susceptibility testing and time-kill assays, and heart infusion agar was used for the enumeration of viable cells.
TP0586532 was synthesized by the Department of Medicinal Chemistry, Taisho Pharmaceutical Co., Ltd. (Saitama, Japan). Amikacin disulfate salt, colistin sulfate, piperacillin, tigecycline hydrate, and azithromycin dihydrate were purchased from Sigma-Aldrich (St. Louis, MO). Cefepime hydrochloride was purchased from US Pharmacopeia (Rockville, MD). Meropenem trihydrate were purchased from US Pharmacopeia or FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Ciprofloxacin hydrochloride monohydrate was purchased from FUJIFILM Wako Pure Chemical Corporation.
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TP0586532 was synthesized by the Department of Medicinal Chemistry, Taisho Pharmaceutical Co., Ltd. (Saitama, Japan). Amikacin disulfate salt, colistin sulfate, piperacillin, tigecycline hydrate, and azithromycin dihydrate were purchased from Sigma-Aldrich (St. Louis, MO). Cefepime hydrochloride was purchased from US Pharmacopeia (Rockville, MD). Meropenem trihydrate were purchased from US Pharmacopeia or FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Ciprofloxacin hydrochloride monohydrate was purchased from FUJIFILM Wako Pure Chemical Corporation.
7
Antibiotic Procurement for Research
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Several antibiotics like amikacin (Lot 01774), aztreonam (Lot MKBW 2997 V), ceftazidime (Lot 1,004,245), ertapenem (Lot T024320) imipenem (Lot 1238-1), meropenem (Lot 037M4713), polymyxin B sulfate (Lot 027M400), tigecycline hydrate (Lot 0000023817), and cinnamaldehyde ≥ 95% (Lot MKBV4784V), were purchased from Sigma-Aldrich, St Louis, MO, USA.
8
Antimicrobial Susceptibility Testing of Klebsiella and E. coli
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K. pneumoniae and E. coli strains were purchased from the American Type Culture Collection (Manassas, VA) or the National Collection of Type Cultures (London, United Kingdom). Cation-adjusted Mueller-Hinton broth was used for antibacterial susceptibility testing and time-kill assays, and heart infusion agar was used for the enumeration of viable cells.
TP0586532 was synthesized by the Department of Medicinal Chemistry, Taisho Pharmaceutical Co., Ltd. (Saitama, Japan). Amikacin disulfate salt, colistin sulfate, piperacillin, tigecycline hydrate, and azithromycin dihydrate were purchased from Sigma-Aldrich (St. Louis, MO). Cefepime hydrochloride was purchased from US Pharmacopeia (Rockville, MD). Meropenem trihydrate were purchased from US Pharmacopeia or FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Ciprofloxacin hydrochloride monohydrate was purchased from FUJIFILM Wako Pure Chemical Corporation.
+ Expand
TP0586532 was synthesized by the Department of Medicinal Chemistry, Taisho Pharmaceutical Co., Ltd. (Saitama, Japan). Amikacin disulfate salt, colistin sulfate, piperacillin, tigecycline hydrate, and azithromycin dihydrate were purchased from Sigma-Aldrich (St. Louis, MO). Cefepime hydrochloride was purchased from US Pharmacopeia (Rockville, MD). Meropenem trihydrate were purchased from US Pharmacopeia or FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Ciprofloxacin hydrochloride monohydrate was purchased from FUJIFILM Wako Pure Chemical Corporation.
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