Total cell extracts were obtained using lysis buffer containing 150 mM Tris-HCl (pH 6.8), 6% SDS, 30% glycerol, and 0.03% Bromophenol Blue; 10% 2-ME was added immediately before harvesting cells. Cell lysates were fractionated on 7.5% SDS-PAGE, transferred to Immobilon-P membranes (Millipore), and incubated with specific antibodies. Western Lightning plus-ECL (PerkinElmer) was used for detection. NFATc1 antibody (556602, 1:1000) was from BD Biosciences; Blimp1 (sc-47732, 1:1000), IRF8 (sc-6058, 1:1000) and GAPDH (sc-25778, 1:3000) antibodies were from Santa Cruz Biotechnology.
Nfatc1 antibody 556602
Manufactured by BD
The NFATc1 antibody (556602) is a laboratory reagent used for detecting and analyzing the presence of the NFAT (nuclear factor of activated T cells) transcription factor c1 subunit in various biological samples. The antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and flow cytometry to study the expression and localization of NFATc1in different cell types and experimental conditions.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh sc 25778, by Santa Cruz Biotechnology (2 mentions)
Immobilon p membrane, by Merck Group (2 mentions)
Western lightning plus ecl, by PerkinElmer (2 mentions)
Irf8 sc 6058, by Santa Cruz Biotechnology (1 mentions)
Sc 535, by Santa Cruz Biotechnology (1 mentions)
Blimp1 sc 47732, by BD (1 mentions)
Pkr sc 6282, by Santa Cruz Biotechnology (1 mentions)
2 protocols using nfatc1 antibody 556602
1
Protein Expression Analysis by Western Blot
2
Western Blot Analysis of Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total cell extracts were obtained using lysis buffer containing 150 mM Tris-HCl (pH 6.8), 6% SDS, 30% glycerol, and 0.03% Bromophenol Blue; 10% 2-ME was added immediately before harvesting cells. Cell lysates were fractionated on 7.5% SDS-PAGE, transferred to Immobilon-P membranes (Millipore), and incubated with specific antibodies. Western Lightning plus-ECL (PerkinElmer) was used for detection. NFATc1 antibody (556602, 1:1000) was from BD Biosciences; Blimp1 (sc-47732, 1:1000), PKR (sc-6282, 1:1000), GAPDH (sc-25778, 1:3000), and p38α (sc-535, 1:3000) antibodies were from Santa Cruz Biotechnology. Densitometry was performed using ImageJ software (http://imagej.nih.gov/ij/ ). The uncropped scans of the western blots are provided in the Supplementary information .
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