1260 infinity g1315c dad

The HPLC system (Agilent Technologies Series 1260, Santa Clara, CA, USA), equipped with a quaternary pump (Agilent Technologies 1260 Infinity G1312B, Santa Clara, CA, USA) and a diode array detector (Agilent Technologies 1260 Infinity G1315C DAD, Santa Clara, CA, USA), was used and adjusted according to the specific conditions of each method, as follows.

Phenolic acids were identified and quantified using an Agilent HPLC system (Agilent Technologies Series 1260, Santa Clara, CA, USA) equipped with a quaternary pump (1260 Infinity G1312B) and a diode array detector (1260 Infinity G1315C DAD). The ZORBAX SB-C18 (3.5 µm, 4.6 × 150 mm) column was used as the stationary phase, and the mobile phase consisted of formic acid 0.1% (solvent A) and acetonitrile (solvent B) at a flow rate of 0.3 mL/min for 50 min. The elution was performed as follows: 0 min 80% A, 5 min 75% A, 15 min 60%, 20 min 45% A, 30 min 35% A, 35 min 35% A, 50 min 80% A. The identification of the phenolic acids was carried out by means of a comparison with the UV spectra, and the retention time of external standards at 280 and 330 nm. Phenolic acids were quantified using the corresponding calibration curve of the external standards and results were expressed as mg/100 g of fresh weight (FW).