Bio plex pro multiplex luminex immunoassays

Serum levels of the following cytokines were tested:  IL-1beta, IL-1ra, IL-6, IL-9, IL-32, RANTES, Eotaxin, IP-10, MIP-1-beta, MCP-1, and Tumor Necrosis Factor-alpha (TNF-α), using Bio-Plex Pro™ multiplex Luminex immunoassays (Bio-Rad Laboratories, Hercules, CA, USA). Testing of these biomarkers was performed at NIS Labs using xMAP technology (Luminex, Austin, TX, USA).

At each visit, a blood draw was performed and used for evaluation of a panel of biomarkers for inflammation and vascular health. The measurement of high sensitivity C-reactive protein was performed at a clinical diagnostic laboratory. Testing of all other biomarkers was performed at NIS Labs using utilizing xMAP technology (Luminex, Austin, TX, USA).
Serum levels of the following cytokines were tested: IL-1β, IL-1ra, IL-6, IL-8 (CXCL8), IL-10, IL-13, IL-17, interferon gamma (IFNγ), MIP-1α, MIP-1β, MCP-1, and tumor necrosis factor alpha (TNFα), using Bio-Plex Pro™ multiplex Luminex immunoassays (Bio-Rad Laboratories, Hercules, CA). Serum levels of 5 matrix metalloproteinases (MMP-1, MMP-2, MMP-7, MMP-9, and MMP-10) and plasma levels of fibrinogen and von Willebrand Factor (vWF) were tested using MilliPlex Luminex panels (Millipore, Burlington, MA USA).