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Glutathione sepharosetm 4b system

Manufactured by GE Healthcare
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The Glutathione SepharoseTM 4B system is a chromatography resin designed for the purification of glutathione-binding proteins. It is composed of glutathione immobilized on cross-linked 4% agarose beads. The system provides a robust and efficient method for the affinity-based separation and recovery of recombinant and native proteins that contain a glutathione S-transferase (GST) tag or possess natural glutathione-binding properties.

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Lab products found in correlation

Pet28a, by Thermo Fisher Scientific (2 mentions) His gravitraptm system, by GE Healthcare (2 mentions) Pgex 4t 1, by Thermo Fisher Scientific (2 mentions)

2 protocols using glutathione sepharosetm 4b system

1

Cloning and Purification of TgTCP-1

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The gene fragment coding for TgTCP-1 was cloned into the pGEX-4T-1 and pET-28a vectors, respectively (Invitrogen, Carlsbad, CA, United States), and the recombinant plasmids were transformed into E. coli BL21 (DE3) for protein expression, respectively. The GST- and His-tagged fusion TgTCP-1 proteins were purified using the Glutathione SepharoseTM 4B system (GE Healthcare) and the His GraviTrapTM system (GE Healthcare), respectively, according to the manufacturer’s instructions. The purified proteins were verified by SDS-PAGE and Western blotting.
Yang N., Xing M., Ding Y., Wang D., Guo X., Sang X., Li J., Li C., Wang Y., Feng Y., Chen R., Wang X., Jiang N, & Chen Q. (2020). The Putative TCP-1 Chaperonin Is an Important Player Involved in Sialic Acid-Dependent Host Cell Invasion by Toxoplasma gondii. Frontiers in Microbiology, 11, 258.
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2

Purification of His- and GST-tagged TatD-like DNase

Cited 1 time
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The genes encoding the His- and GST-tagged TatD-like DNase of P. berghei (PBANKA_0201800) were cloned into the pET-28a and pGEX-4T-1 vectors, respectively (Invitrogen), and expressed in Escherichia coli BL21(DE3), as described in our earlier study (Chang et al., 2016 (link)). His- and GST-tagged recombinant proteins were purified using the His GraviTrapTM system (GE Healthcare) and the Glutathione SepharoseTM 4B system (GE Healthcare), respectively, according to the manufacturer’s instructions. Purified proteins were analyzed with SDS-PAGE and Western blots before further experiments.
Wang W., Liu F., Jiang N., Lu H., Yang N., Feng Y., Sang X., Cao Y, & Chen Q. (2018). Plasmodium TatD-Like DNase Antibodies Blocked Parasite Development in the Mosquito Gut. Frontiers in Microbiology, 9, 1023.
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