Vs 120 l slide scanner 100 w system

In situ reporter fluorescence-stained slides were analyzed on an EVOS^® FL microscope (Thermo Fisher Scientific). Confocal microscopy was performed using the laser scanning microscope Axio Observer Z1 attached to LSM700 (Carl Zeiss, Jena, Deutschland). Light microscopic cell culture pictures were obtained with an EVOS^® XL microscope (Thermo Fisher Scientific). Total slides were scanned automatically in 40 x magnification using the VS-120-L Olympus slide scanner 100-W system and processed using the Olympus VS-ASW-L100 program (Olympus, Shinjuku, Tokio, Japan).

For histochemistry and immune‐histochemistry, paraffin‐embedded skin samples were cut into 4 μm sections. Haematoxylin and eosin (HE) staining using Mayer's Hemalaun (1.09249.2500, Merck) and Eosin Y (1.15935.0100, Merck) was done in a linear slide stainer (Leica ST4040). For Masson–Goldner trichrome staining, kits were used according to the manufacturer's recommendations (12043, 14604, Morhisto). Automatic scanning of full slides in 40x magnification was done using the VS‐120‐L Olympus slide scanner 100‐W system and processed using the Olympus VS‐ASW‐L100 program. Evaluation of the epidermal thickness and murine vessel quantification was done according to published work (Ebner‐Peking et al., 2021 (link)), both measured in the Olympus VS‐ASM‐L100 program. Per group, four biological and at least three technical replicates were included.

Slides were scanned automatically in 20x or 40x magnification using the VS-120-L Olympus slide scanner 100-W system. Pictures were processed using the Olympus VS-ASW-L100 program and figures were prepared using Microsoft PowerPoint 2010.