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White 96 well luminometer plate

Manufactured by Thermo Fisher Scientific
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The white 96-well luminometer plate is a laboratory equipment used to conduct luminescence-based assays. It features a flat surface with 96 individual wells, designed to hold samples for analysis in a luminometer instrument. The plate is made of a white material, which helps optimize light reflection and signal detection during luminescence measurements.

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Lab products found in correlation

Hank s balanced salt solution hbss with mg2 and ca2, by Thermo Fisher Scientific (2 mentions) Horseradish peroxidase hrp type 4, by Merck Group (2 mentions) Dmem without phenol red, by Merck Group (2 mentions) Hanks balanced salt solution (hbss), by Merck Group (2 mentions) Victor nivo, by PerkinElmer (2 mentions) Victor v3, by PerkinElmer (2 mentions)

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96-well plates (1169 citations)

2 protocols using white 96 well luminometer plate

1

Monitoring Intracellular and Extracellular ROS in Immune Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Intra- and extracellular reactive oxygen species (ROS) accumulation was monitored at an MOI of 5:1 using a luminol-dependent chemiluminescence assay as described earlier (Frohner et al., 2009 (link)). Briefly, 4 × 104 BMDMs or neutrophils were suspended in DMEM without phenol red (Sigma-Aldrich) or Hank’s balanced salt solution (HBSS; with Mg2+ and Ca2+; GIBCO) in a well of a white 96-well luminometer plate (Nunc) and mixed with 50 μL HBSS containing 200 μM luminol (intra- and extracellular ROS) or 600 μM isoluminol (extracellular ROS) and 16 U horseradish peroxidase (HRP) type IV (all Sigma-Aldrich). Immediately afterward, 50 μL HBSS containing the indicated C. albicans strains, heat-killed C. albicans, 75 μg Zymosan or 1 μg TDB were added. Chemiluminescence was monitored on a Victor V3 or a Victor Nivo microplate reader (both PerkinElmer). As controls, BMDMs or neutrophils without stimulation and C. albicans alone were included. The detected relative luciferase units (RLU) are expressed as RLU per min per 1000 immune cells or as total RLU after the indicated time.
Jenull S., Mair T., Tscherner M., Penninger P., Zwolanek F., Silao F.G., de San Vicente K.M., Riedelberger M., Bandari N.C., Shivarathri R., Petryshyn A., Chauhan N., Zacchi L.F., LeibundGut -Landmann S., Ljungdahl P.O, & Kuchler K. (2021). The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence. Cell reports, 36(3), 109406.
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2

Monitoring Intracellular and Extracellular ROS in Immune Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Intra- and extracellular reactive oxygen species (ROS) accumulation was monitored at an MOI of 5:1 using a luminol-dependent chemiluminescence assay as described earlier (Frohner et al., 2009 (link)). Briefly, 4 × 104 BMDMs or neutrophils were suspended in DMEM without phenol red (Sigma-Aldrich) or Hank’s balanced salt solution (HBSS; with Mg2+ and Ca2+; GIBCO) in a well of a white 96-well luminometer plate (Nunc) and mixed with 50 μL HBSS containing 200 μM luminol (intra- and extracellular ROS) or 600 μM isoluminol (extracellular ROS) and 16 U horseradish peroxidase (HRP) type IV (all Sigma-Aldrich). Immediately afterward, 50 μL HBSS containing the indicated C. albicans strains, heat-killed C. albicans, 75 μg Zymosan or 1 μg TDB were added. Chemiluminescence was monitored on a Victor V3 or a Victor Nivo microplate reader (both PerkinElmer). As controls, BMDMs or neutrophils without stimulation and C. albicans alone were included. The detected relative luciferase units (RLU) are expressed as RLU per min per 1000 immune cells or as total RLU after the indicated time.
Jenull S., Mair T., Tscherner M., Penninger P., Zwolanek F., Silao F.G., de San Vicente K.M., Riedelberger M., Bandari N.C., Shivarathri R., Petryshyn A., Chauhan N., Zacchi L.F., LeibundGut -Landmann S., Ljungdahl P.O, & Kuchler K. (2021). The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence. Cell reports, 36(3), 109406.
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