Cdn1163

Manufactured by Merck Group

Sourced in United States

CDN1163 is a piece of laboratory equipment manufactured by Merck Group. It is designed for general laboratory use, but its core function is not explicitly stated.

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Lab products found in correlation

5 protocols using cdn1163

Allosteric SERCA Activator CDN1163 in mdx Mice

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CDN1163, an allosteric SERCA activator, was purchased from Sigma-Aldrich (St. Louis, MO). CDN1163 was reported to be selective against > 160 off-targets (23 ). CDN1163 showed an acceptable pharmacokinetic profile in mice (20 (link),21 (link)), and membranes showed high permeability to CDN1163. Vehicle (10% DMSO, 10% Tween-80 in PBS) or CDN1163 (1.0 mg/ml stock solution, final dose of 40 mg/kg) was intraperitoneally injected into mdx mice.

Nogami K., Maruyama Y., Sakai-Takemura F., Motohashi N., Elhussieny A., Imamura M., Miyashita S., Ogawa M., Noguchi S., Tamura Y., Kira J.I., Aoki Y., Takeda S, & Miyagoe-Suzuki Y. (2021). Pharmacological activation of SERCA ameliorates dystrophic phenotypes in dystrophin-deficient mdx mice. Human Molecular Genetics, 30(11), 1006-1019.

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Calcium Signaling Pathway Analysis

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Phospho MLC₂₀ (Ser19), total MLC₂₀, β-actin antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). SERCA2a and SERCA2b antibodies were purchased from Badrilla Ltd (Leads, UK). Fura-2am, Fura-2am calibration kit, Alexa Fluor secondary antibodies, DMEM/F12, DMEM, fetal bovine serum (FBS), triple antibiotics (penicillin, streptomycin, and Amphotericin B), Prolong Gold antifade mounting medium with DAPI, WGA dye, and Alexa Flour 488 antibody labeling kit were purchased from Thermo Fischer Scientific (Waltham, MA, USA). SEC61A antibody was purchased from Abcam (Cambridge, MA, USA). DMSO, thapsigargin, CDN1163, bovine serum albumin, and α-SMA antibody were purchased Sigma Aldrich (St Louis, MO, USA). All other chemicals and reagents were from Sigma Aldrich (St. Louis, MO, USA), otherwise we indicated specifically.

Lee Y., Chakraborty S, & Muthuchamy M. (2020). Roles of sarcoplasmic reticulum Ca2+ ATPase pump in the impairments of lymphatic contractile activity in a metabolic syndrome rat model. Scientific Reports, 10, 12320.

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Detailed Reagent Procurement Protocol

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All chemicals used in this study were purchased at reagent quality (purity > 95% by high-performance liquid chromatography): CDN1163, N-(2-methylquinolin-8-yl)-4-propan-2-yloxybenzamide (Sigma, St. Louis, MO, USA); istaroxime, (3E,5S,8R,9S,10R,13S,14S)-3-(2-aminoethoxyimino)-10,13-dimethyl-1,2,4,5,7,8,9,11,12,14,15,16-dodecahydrocyclopenta[a]phenanthrene-6,17-dione (MedChemExpress LLC, Monmouth Junction, NJ, USA); CP-154526, N-butyl-N-ethyl-2,5-dimethyl-7-(2,4,6-trimethylphenyl)pyrrolo[2,3-d]pyrimidin-4-amine (Sigma), Ro 41-0960, (3,4-dihydroxy-5-nitrophenyl)-(2-fluorophenyl) methanone (Sigma); AMP-PCP, β, γ-methyleneadenosine-5′-triphosphate (Sigma).

Šeflová J., Cruz-Cortés C., Guerrero-Serna G., Robia S.L, & Espinoza-Fonseca L.M. (2023). Mechanisms for cardiac calcium pump activation by its substrate and a synthetic allosteric modulator using fluorescence lifetime imaging. PNAS Nexus, 3(1), pgad453.

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Comprehensive TMAO Metabolism Analysis

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TMAO (Sigma, Cat. 317594), D9-TMAO(Cambridge Isotopes,Cat. DML-4779-1), [6]-gingerol (Sigma, Cat. 345868), CDN1163 (Sigma, Cat. SML1682), MCC950 sodium (MCE, Cat. HY-12815A), Glucose (Sinopharm Chemical Reagent Co., Ltd. Cat. 10010592), GLP-1 (7-36) (MCE, Cat. HY-P0054), L-Arginine monohydrochloride (Sigma, Cat. A5131), Fatty acid free bovine serum albumin (Equitech-Bio, Inc.Cat. BAH66), Collagenase from Clostridium histolytium (Sigma, Cat. C9263), Human recombinant insulin (Lilly Cat. HI0219), Fura-2, AM (Invitrogen, Cat. F1221), mitoSOX Red, Molecular Probes (Invitrogen, M36008), phosphoenolpyruvate (Sigma, Cat. P7127), ATP disodium salt (Sigma, Cat. A3377), pyruvate kinase (Sigma, P1903-1KU), lactate dehydrogenase (Sigma, Cat. 59747), NADH (Sigma, Cat. N8129), digitonin (Sigma, Cat. D141), protease inhibitor cocktail (Sigma, Cat. P8340), PMSF (Roche, Cat. 10837091001), 4-Bromo A23187 (MCE, Cat. HY-N6694), Anti-Biotin MicroBeads (Miltenyi Biotec, Cat. 130-090-485).

Kong L., Zhao Q., Jiang X., Hu J., Jiang Q., Sheng L., Peng X., Wang S., Chen Y., Wan Y., Hou S., Liu X., Ma C., Li Y., Quan L., Chen L., Cui B, & Li P. (2024). Trimethylamine N-oxide impairs β-cell function and glucose tolerance. Nature Communications, 15, 2526.

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Modulating SERCA Activity in HEK293T Cells

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HEK293T cells were transfected as indicated in Table 2. After 18-hour incubation wrapped in foil in a 37°C/5% CO₂ incubator, media was replaced with 100µL complete DMEM +/− 30µM SERCA activator CDN1163 (Sigma-Aldrich) and incubated at 37°C for 1 hour. Then, media was replaced again with 100µL DMEM + 10% FBS + 1% PS containing 10µM furimazine and one of the following SERCA inhibitors: 10µM cyclopiazonic acid (CPA) (Sigma-Aldrich), 10µM 2,5-di-(tert-butyl)-1,4-hydroquinone (DBHQ) (Sigma-Aldrich), or 10µM Thapsigargin (EMD Millipore). Bioluminescence measurements were taken in real-time immediately after adding SERCA inhibitor + furimazine and compared to cells treated with just 10µM furimazine. Firefly luciferase reporter expression was measured ~12 hours later, as described above.

Seto S., Layh-Schmitt G., Kenri T, & Miyata M. (2001). Visualization of the Attachment Organelle and Cytadherence Proteins of Mycoplasma pneumoniae by Immunofluorescence Microscopy. Journal of Bacteriology, 183(5), 1621-1630.

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