Macs magnetic separation system

Manufactured by Miltenyi Biotec

Sourced in United States

The MACS magnetic separation system is a versatile tool for the isolation and purification of specific cell populations from complex biological samples. It utilizes magnetic beads coated with antibodies or ligands to target and capture desired cells, which are then isolated through a magnetic field. The system enables efficient and gentle separation, making it suitable for a wide range of applications in cell biology, immunology, and stem cell research.

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Lab products found in correlation

Dextran, by Thermo Fisher Scientific (2 mentions) Navios, by Beckman Coulter (1 mentions) Monocyte isolation kit, by Miltenyi Biotec (1 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) Gm csf, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharide from e coli 055 b5, by Merck Group (1 mentions) 6 well plate, by Greiner (1 mentions) Leupeptin, by Cayman Chemical (1 mentions) Mda mb 231 cell, by American Type Culture Collection (1 mentions) Ficoll, by Corning (1 mentions) Thp 1, by American Type Culture Collection (1 mentions) Ecl detection kit, by Merck Group (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Merck Group (1 mentions) Aprotinin, by Cayman Chemical (1 mentions) Protease inhibitor cocktail tablet, by Roche (1 mentions) Palmostatin b, by Merck Group (1 mentions)

3 protocols using macs magnetic separation system

Enriching Human Melanoma Cells

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In this study, we used the anti-Cluster Differentiation 90 Monoclonal Antibody (Anti-CD90) to identify and quantify fibroblasts in melanoma primary cultures by flow cytometry (FACS; Beckman Coulter Navios).
Fibroblasts (CD90+ cells) depletion of primary melanoma cultures was performed by applying the MACS magnetic separation system (Miltenyi Biotec). Briefly, cells were magnetically labeled with CD90-MicroBeads and loaded into a MACS^® Column (type LD), which was placed in the magnetic field of a MACS Separator. Fibroblasts (CD90+) were retained within the column while melanoma cells (unlabeled cells) run through.
This operation was repeated allowing us to highly enriched the population of human melanoma cells and get rid of fibroblasts.

Najem A., Wouters J., Krayem M., Rambow F., Sabbah M., Sales F., Awada A., Aerts S., Journe F., Marine J.C, & Ghanem G.E. (2021). Tyrosine-Dependent Phenotype Switching Occurs Early in Many Primary Melanoma Cultures Limiting Their Translational Value. Frontiers in Oncology, 11, 780654.

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Monocyte-Derived Dendritic Cell Assay

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Example 25

Monocytes were isolated from cryopreserved PBMCs by negative selection methods using a Monocyte Isolation Kit and the MACS™ magnetic separation system (Miltenyi Biotec). Monocytes were resuspended in RPMI 1640 medium containing 10% hiFBS and 100 ng/mL GM-CSF and IL-4 (both Peprotech). Cells were cultured for 5 days in non-TC treated 6-well plates (Greiner) to induce differentiation of DCs, before addition of 100 ng/mL lipopolysaccharide from E. coli 055:B5 (Sigma) to activate the DCs. Cells were harvested after 24 hours of activation, and washed once with PBS to remove LPS, and resuspended at 10⁶/mL in RPMI 10% hiFBS. Allogeneic CD3⁺ T-cells were isolated from cryopreserved PBMC using a Pan T-Cell Isolation kit and the MACS system as above, and resuspended at 2×10⁶/mL in RPMI 10% hiFBS. Serial dilutions of selected antibodies (1:3) from 10 nM were prepared in RPMI 10% hiFBS and 50 μL added to 96-well, flat-bottomed TC plates plates in triplicate. DCs (100 μL) and T-cells (50 μL) were added to plates and incubated at 37° C., 5% C02 for five days. Supernatants were removed after three days for measurement of IL-2, and five days for measurement of IFNγ. Supernatants were stored at −20° C. until use. Cytokine production was measured with the R&D Systems Human IFNγ and IL-2 Duoset® ELISA, using DELFIA® Eu-N1 Streptavidin detection. Results are shown in FIG. 24.

US11440960B2. TIGIT antibodies, encoding nucleic acids and methods of using said antibodies in vivo (2022-09-13). Kymab Limited [GB]. Inventors: Joana de Abreu Carvalho [GB], Rachael Jane Kimber [GB], Jamie Ian Campbell [GB], Nikole Sandy [GB], Cassandra Van Krinks [GB], Stephen John Arkinstall [GB], Volker Germaschewski [GB], Ian Kirby [GB], Miha Kosmac [GB], Thomas Gallagher [GB], Matthew John McCourt [GB], Richard Charles Alfred Sainson [GB], Mohammed Hanif Ali [GB], E-Chiang Lee [GB], Stephen Douglas Gillies [US].

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Isolation and Analysis of Lipid Metabolizing Enzymes

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HBSS, RPMI, 1640 Leibovitz's L-15 medium, trypsin, and FBS were obtained from Wisent Laboratories (St-Bruno, Quebec City, Canada). Ficoll was obtained from Corning (Tewksbury, MA, USA). Dextran was purchased from Fisher Scientific (Ottawa, ON, Canada). The CD14 and CD16 magnetic bead-coupled antibody and the MACS magnetic separation system were purchased from Miltenyi Biotec (Auburn, CA, USA). THP-1 and MDA-MB-231 cells were obtained from ATCC (Manassas, VA, USA). Human hypothalamus samples were provided by the Douglas-Bell Canada Brain Bank (Montréal, Québec, Canada). Protease inhibitor cocktail tablets were purchased from Roche (Laval, QC, Canada). Aprotinin, leupeptin, and 4′-[ [[methyl[[3-(4-pyridinyl) [1,1′-biphenyl]-4-yl ester carbamic acid (WWL70), as well as the primary antibodies for MAG lipase, were purchased from Cayman Chemical (Ann Arbor, MI, USA). The LYPLA2 primary antibody, the ABHD16A primary antibody, Dextran and the fluorophosphonate (FP)-TAMRAprobewere from Thermo Fisher Scientific (Waltham, MA, USA) and the primary antibody for carboxylesterase (CES)1 was from R&D Systems (Minneapolis, MN, USA). PMSF, Palmostatin B, and the ECL detection kit were from EMD Millipore (Billerica, MA, USA). ML349 was a generous gift from Dr. Lawrence J. Marnett (Vanderbilt University, Nashville, TN, USA).

Turcotte C., Dumais É., Archambault A.S., Martin C., Blanchet M.R., Bissonnette É., Boulet L.P., Laviolette M., Di Marzo V, & Flamand N. (2019). Human leukocytes differentially express endocannabinoid-glycerol lipases and hydrolyze 2-arachidonoyl-glycerol and its metabolites from the 15-lipoxygenase and cyclooxygenase pathways. Journal of leukocyte biology, 106(6).

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