Sea0400

Manufactured by Bio-Techne

Sourced in United Kingdom

SEA0400 is a selective inhibitor of the sodium-calcium exchanger (NCX) protein. It is a small molecule compound that specifically targets and blocks the activity of the NCX, which is involved in regulating calcium homeostasis in cells.

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Lab products found in correlation

Fura 2 am, by Thermo Fisher Scientific (1 mentions) Terazosin, by Bio-Techne (1 mentions) Metoprolol, by Bio-Techne (1 mentions) Clozapine n oxide cno, by Enzo Life Sciences (1 mentions) Collagenase type 2, by Thermo Fisher Scientific (1 mentions)

3 protocols using sea0400

Fura-2/AM and SEA0400 Protocol

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Fura-2/AM was obtained from Molecular Probes (Molecular Probes Europe BV, Leiden, The Netherlands). SEA0400 was obtained from Tocris Bioscience (Bristol, UK). All other chemicals were purchased from Sigma (Sigma-Aldrich Quimica, Toluca, Estado de México, México).

Berra-Romani R., Guzmán-Silva A., Vargaz-Guadarrama A., Flores-Alonso J.C., Alonso-Romero J., Treviño S., Sánchez-Gómez J., Coyotl-Santiago N., García-Carrasco M, & Moccia F. (2019). Type 2 Diabetes Alters Intracellular Ca2+ Handling in Native Endothelium of Excised Rat Aorta. International Journal of Molecular Sciences, 21(1), 250.

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Pharmacological Inhibition Assay Protocol

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All standard chemicals were purchased from Sigma-Aldrich unless mentioned otherwise. CNQX (6-cyano-7-nitro-quinoxaline-2,3-dione, Sigma), clozapine-N-oxide (CNO) (Enzo Life Sciences), APV (D-2-amino-5-phosphono-pentanoic acid, Sigma), MPEP (6-(phenylethynyl)-pyridine, Tocris), terazosin (Tocris), metoprolol (Tocris), SN-6 (2-[4-(4-nitrobenzyloxy)benzyl]thiazolidine-4-carboxylic acid ethyl ester, Tocris), SEA0400 (2-[4-[(2,5-difluorophenyl)methoxy]-phenoxy]-5-ethoxyaniline, CM-4620 (Tocris), synthesized by Taisyo Pharmaceutical Co. Ltd), UTP (uridine 5’-triphosphate, Tocris).

Wang F., Wang W., Gu S., Qi D., Smith N.A., Peng W., Dong W., Yuan J., Zhao B., Mao Y., Cao P., Lu Q.R., Shapiro L.A., Yi S.S., Wu E, & Huang J.H. (2023). Distinct astrocytic modulatory roles in sensory transmission during sleep, wakefulness, and arousal states in freely moving mice. Nature Communications, 14, 2186.

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SEA0400 Compound Extraction Protocol

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SEA0400 was obtained from Tocris Bioscience (Bristol, United Kindom). Collagenase type 2 and FBS were obtained from Gibco (GIBCO BRL, Life Technologies, Grand Island, NY). All other chemicals were purchased from Sigma-Aldrich.

Moreno-Salgado A., Coyotl-Santiago N., Moreno-Vazquez R., Lopez-Teyssier M., Garcia-Carrasco M., Moccia F, & Berra-Romani R. (2023). Alterations of the Ca2+ clearing mechanisms by type 2 diabetes in aortic smooth muscle cells of Zucker diabetic fatty rat. Frontiers in Physiology, 14, 1200115.

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