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Lineage cell depletion kit for mice

Manufactured by Miltenyi Biotec
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Sourced in Spain

The Lineage Cell Depletion Kit for mice is a laboratory tool designed for the depletion of specific cell lineages from mouse samples. The kit utilizes a combination of antibodies and magnetic beads to selectively remove unwanted cell populations, enabling the enrichment of target cell types for further analysis or experimentation.

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Lab products found in correlation

Cd16 32, by Thermo Fisher Scientific (1 mentions) Gotaq r qpcr master mix, by Promega (1 mentions) Rnaseout ribonuclease inhibitor, by Thermo Fisher Scientific (1 mentions) Anti sca 1, by Miltenyi Biotec (1 mentions) Anti gr1, by Miltenyi Biotec (1 mentions) Anti ter 119, by Miltenyi Biotec (1 mentions) Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (1 mentions) Tri reagent, by Merck Group (1 mentions) Fcr blocking reagent, by Miltenyi Biotec (1 mentions)

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Lineage depletion kit

2 protocols using lineage cell depletion kit for mice

1

Murine Hematopoietic Cell Analysis

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TRI reagent was from Sigma-Aldrich (Madrid, Spain). Antibodies for the detection of murine CD135 (Flt3), CD34, CD16/32, and CD127 (IL-7Rα) were from eBioscience (Barcelona, Spain). The Lineage Cell Depletion Kit for mice, FcR Blocking reagent and murine flow cytometry antibodies (CD3e, CD4, CD8a, CD11b, CD19, CD43, CD45R (B220), CD117 (c-Kit), anti-Gr1, anti-Sca-1, anti-Ter-119, anti-IgM) were from Miltenyi Biotec (Madrid, Spain). Super Script II reverse transcriptase and RNase OUT ribonuclease inhibitor were from Invitrogen and Thermo Fisher Scientific (Madrid, Spain). GoTaqR qPCR master mix was from Promega (Madrid, Spain).
Prieto-Bermejo R., Romo-González M., Pérez-Fernández A., García-Macías M.C., Sánchez-Bernal C., García-Tuñón I., Sánchez-Yagüe J., Sánchez-Martín M, & Hernández-Hernández Á. (2021). Granuloma Formation in a Cyba-Deficient Model of Chronic Granulomatous Disease Is Associated with Myeloid Hyperplasia and the Exhaustion of B-Cell Lineage. International Journal of Molecular Sciences, 22(16), 8701.
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2

Enrichment and Lentiviral Transduction of Murine BM Cells

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BM cells were flushed with RPMI medium from femurs and tibias. Preparations were filtered to remove debris and washed twice in phosphate-buffered saline. Lin– cells were purified with the Lineage Cell Depletion Kit for mice (MiltenyiBiotec), according to the manufacturer’s instructions. Lin– cell lentiviral transduction was performed as described (Sardina et al., 2014 (link)).
López-Ruano G., Prieto-Bermejo R., Ramos T.L., San-Segundo L., Sánchez-Abarca L.I., Sánchez-Guijo F., Pérez-Simón J.A., Sánchez-Yagüe J., Llanillo M, & Hernández-Hernández Á. (2015). PTPN13 and β-Catenin Regulate the Quiescence of Hematopoietic Stem Cells and Their Interaction with the Bone Marrow Niche. Stem Cell Reports, 5(4), 516-531.
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