For cytotoxic study of mushroom fruit body, aqueous cold extraction was done. In this extraction, 20 g of air-dried pieces of mushroom were powdered in Mixer Grinder (Philips HL1606/03 500 W, HP, India) and dissolved in 100 mL of distilled water followed by incubation in orbital shaker (Metrex, New Delhi, India) for 24 h at 25 ± 2 °C. This aqueous mixture of mushroom centrifuged at 5000 rpm for 15 min at 4 °C, supernatant was collected and filter by Whatman filter paper No. 1 (125 mm). The filtrate kept on rotatory evaporator (BUCHI, B-300, Mumbai, India) for the evaporation of water until the extract is appear like semisolid gel. This final extract was stored at 4 °C until further use.
Whatman filter paper no 1 125 mm
Manufactured by Cytiva
Whatman filter paper No. 1 (125 mm) is a general-purpose filter paper designed for a variety of laboratory filtration applications. It is made from high-quality cellulose and offers consistent performance and reliability.
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Lab products found in correlation
2 protocols using whatman filter paper no 1 125 mm
1
Cytotoxic Mushroom Extraction Protocol
2
Ethanolic Leaf Extract Preparation
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Cold maceration method was adopted in the preparation of the leaf extracts. Exactly 50 g each of the powdered leaves was soaked in 500 mL of 80% ethanol (w/v) kept and kept for 72 hours in a tightly sealed plastic container at room temperature. Filtration was done with muslin cloth and later re-filtered with Whatman filter paper No 1(125 mm). The wet extract was concentrated into pasty form using a crude method for bacterial susceptibility test experiment.
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