Sp sepharose ff

Manufactured by Cytiva

SP-Sepharose FF is a strong cation exchange media used for the purification and separation of biomolecules. It is composed of cross-linked agarose beads with covalently attached sulfopropyl functional groups. SP-Sepharose FF can be used for the purification of proteins, peptides, and other charged biomolecules.

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Lab products found in correlation

Histrap hp column, by Cytiva (1 mentions) Kanamycin, by Merck Group (1 mentions) Superdex 200, by Cytiva (1 mentions) Q sepharose ff, by Cytiva (1 mentions) Isopropylthiogalactopyranoside iptg, by Merck Group (1 mentions) 12 o tetradecanoylphorbol 13 acetate pma, by Merck Group (1 mentions)

2 protocols using sp sepharose ff

Recombinant Protein Expression in E. coli

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E. coli strain
BL21(DE3) pLysS and the pET28a expression vector were obtained from
Novagen Inc. (Madison, WI). Amicon centrifugal filter devices (3000 M_r cutoff) were purchased from Millipore (Bedford,
MA). Q-Sepharose FF, SP-Sepharose FF, Superdex-200, and His-Trap HP
columns were obtained from Amersham Biosciences. Kanamycin, isopropyl
thiogalactopyranoside (IPTG), and 12-O-tetradecanoylphorbol-13-acetate
(PMA) were obtained from Sigma. Human α-thrombin (IIa), FXIa, pKLK, and plasmin were purchased from Haematologic Technologies
Inc. tPA (Alteplase) was purchased from Genentech (South San Francisco,
CA). uPA was obtained from Calbiochem, EMD Biosciences Inc. (San Diego,
CA). Glu–Plg was obtained from Enzyme Research Laboratories
(South Bend, IN). εACA (Amicar) was obtained from ICN Biomedicals
Inc. (Aurora, OH). Normal pooled plasma (NPP) was purchased from George
King Bio-Medical Inc. (Overland Park, KS). Plasmin substrate S-2251
(H-d-Val-Leu-Lys-p-nitroanilide) and pKLK and FXIa substrate S-2366 (pyroGlu-Pro-Arg-p-nitroanilide) were obtained from Diapharma Inc. All other
reagents were of the highest purity commercially available.

Kumar Y., Vadivel K., Schmidt A.E., Ogueli G.I., Ponnuraj S.M., Rannulu N., Loo J.A., Bajaj M.S, & Bajaj S.P. (2014). Decoy Plasminogen Receptor Containing a Selective Kunitz-Inhibitory Domain. Biochemistry, 53(3), 505-517.

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Purification of Ub::hGH Protein

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The pooled Ub::hGH peak fractions from the DEAE Sepharose FF column were directly loaded onto a 30-ml column filled with SP Sepharose FF (Amersham Pharmacia Biotech AB) equilibrated with 6–8 M urea and 20 mM phosphate buffer at pH 7.0. The flow through material, containing the Ub::hGH protein, was collected and renatured. Separation on this column was not necessary if the Ub::hGH protein yield from the DEAE column was about 70%.

Wojtowicz-Krawiec A., Sokolowska I., Smorawinska M., Chojnacka-Puchta L., Mikiewicz D., Lukasiewicz N., Marciniak-Rusek A., Wolinowska R., Bierczynska-Krzysik A., Porebska A.J., Kuthan-Styczen J., Gurba L., Borowicz P., Mazurkiewicz A., Plucienniczak G, & Plucienniczak A. (2014). Use of Ubp1 protease analog to produce recombinant human growth hormone in Escherichia coli. Microbial Cell Factories, 13, 113.

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