Recql1 a300 450a

Manufactured by Fortis Life Sciences

Sourced in China

RECQL1 (A300-450A) is a laboratory product for research purposes. It is a protein that plays a role in DNA repair processes. The product can be used by researchers to study the function and characteristics of the RECQL1 protein, but no further details about its intended use or applications are provided.

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Lab products found in correlation

Usp28 17707 1 ap, by Proteintech (2 mentions) Wrn 4666s, by Cell Signaling Technology (2 mentions) Blm a300 110a, by Fortis Life Sciences (2 mentions) Flag f3165, by Merck Group (1 mentions) C myc sc 40, by Santa Cruz Biotechnology (1 mentions) γh2ax 05 636, by Merck Group (1 mentions) Ub sc 8017, by Santa Cruz Biotechnology (1 mentions) Phospho chk1 ser345 2348, by Cell Signaling Technology (1 mentions) Gapdh 60004 1 1g, by Proteintech (1 mentions) Protein a agarose beads, by GE Healthcare (1 mentions) Benzonase, by Merck Group (1 mentions)

3 protocols using recql1 a300 450a

Antibody Characterization for Helicase Enzymes

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The antibodies used in this study were as follows: USP28 (17707-1-AP, 1:1000 WB, Proteintech); RECQL1 (A300-450A, 1:1000 WB, Bethyl Lab, Montgomery); WRN (4666S,1:1000 WB,Cell Signaling); BLM (A300-110A,1:1000 WB, Bethyl Lab); RECQL4 (17008-1-AP,1:1000 WB, Proteintech); RECQL5 (A302-520A, 1:2000 WB, Bethyl Lab); Flag (F3165, 1:2000, Sigma); Ub (SC-8017, 1:200 WB, Santa Cruz Biotechnology); γH2AX (05-636, 1:500 IF, Millipore); Claspin (2800S, 1:1000 WB, Cell Signaling); c-MYC (SC-40, 1:200 WB, Santa Cruz); Phospho-Chk1-Ser345 (2348, 1:1000 WB, Cell Signaling); CHK1 (ab32531, 1:1000 WB, Abcam); GAPDH (60004-1-1g, 1:5000 WB, Proteintech); Tubulin (66240-1-1g, 1:5000 WB, Proteintech); ACTIN (66009-1-1g, 1:3000 WB, Proteintech). The secondary antibodies conjugated to horseradish peroxidase for Western blot and the secondary antibodies anti-mouse, -goat or -rabbit Alexa fluor 488 or 594 for immunofluorescence staining were purchased from Jackson ImmunoResearch Laboratories. siRNAs were synthesized by GenePharma.

Wang J., Dong Y., Ma H., Wu L., Zhen X., Tang L., Jin J., Han S., Zhang P, & Peng J. (2021). The deubiquitinase USP28 stabilizes the expression of RecQ family helicases and maintains the viability of triple negative breast cancer cells. The Journal of Biological Chemistry, 298(1), 101443.

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Immunoblotting and Co-immunoprecipitation of DNA Damage Proteins

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Whole-cell extracts were prepared and subjected to immunoblotting as previously described (23 (link)). Immunoblotting was performed using antibodies against pS1981 ATM (AF1655) from R&D Systems; ATM (A300-299A), pS824-KAP1 (A300-767A), KAP1 (A300-274A), pS966-SMC1 (A300-050A), SMC1 (A300-055A), pS4/S8-RPA2 (A300-245A), and RECQL1 (A300-450A) from Bethyl Laboratories; pS345-CHK1 (2341) from Cell Signaling Technology; CHK1 (sc-8408) and PARP1 (sc-8007) from Santa Cruz Biotechnology; H2A (07-146), γ-H2AX (05-636), RPA2 (NA18), and HA (H9658) from Merck; pS343-NBS1 (47272) from Abcam; and Nbs1 (GTX70224) from GeneTex. Co-immunoprecipitation was carried out as previously described (23 (link)), with the exception that EDTA was excluded, and 250 units/mL benzonase (Merck) and 1.5 mM MgCl₂ were added to the lysis buffer. Anti-RECQL1 antibody (5 μg, A300-450A) or a nonspecific IgG (Dako) coupled with protein A agarose beads (GE Healthcare) were used to isolate protein complexes from 5 mg whole-cell extract.

Abu-Libdeh B., Jhujh S.S., Dhar S., Sommers J.A., Datta A., Longo G.M., Grange L.J., Reynolds J.J., Cooke S.L., McNee G.S., Hollingworth R., Woodward B.L., Ganesh A.N., Smerdon S.J., Nicolae C.M., Durlacher-Betzer K., Molho-Pessach V., Abu-Libdeh A., Meiner V., Moldovan G.L., Roukos V., Harel T., Brosh RM J.r, & Stewart G.S. (2022). RECON syndrome is a genome instability disorder caused by mutations in the DNA helicase RECQL1. The Journal of Clinical Investigation, 132(5), e147301.

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Western Blot Antibodies for DNA Repair

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The antibodies used in this study were as follows: USP28 (17707-1-AP, 1:1000 WB, Proteintech, Wuhan, China); RECQL1 (A300-450A, 1:1000 WB, Bethyl Lab, Montgomery, TX, USA); WRN ( 4666S,1:1000 WB ,Cell Signaling, Beverly, MA, USA); BLM (A300-110A ,1:1000 WB, Bethyl Lab); RECQL4 (17008-1-AP ,1:1000 WB, Proteintech); RECQL5 (A302-520A, 1:2,000 WB, Bethyl Lab); Flag (F3165,

Peng J., Wang J., Dong Y., Ma H., Wu L., Zhen X., Tang L., Jin J., Han S., & Zhang P. (2021). The Deubiquitin Enzyme USP28 Maintains the Viability of Triple Negative Breast Cancer Cells through Stabilizing the RecQ Family Helicases.

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