All FACS antibodies (Abs) and blocking Abs were purchased from BD Biosciences or eBioscience. Mouse anti β-actin monoclonal antibody (mAb), rabbit anti-Tiam1 antibody, and mouse anti-Rac1 antibody used for western blot were purchased from Sigma-Aldrich, Millipore and BD Biosciences, respectively. Mouse monoclonal Tiam1 antibody from Santa Cruz Biotechnology was used for immunoprecipitation. Other antibodies used for co-IP include mouse anti RORγ (BD Biosciences), mouse anti-Rac1 (Abcam), mouse anti lamin A/C (Cell Signaling) and goat anti paxillin (Santa Cruz Biotechnology). For the proximal ligation assay, rabbit anti-Tiam1 (Santa Cruz Biotechnology), mouse anti-Rac1 (Abcam), mouse anti RORγ (BD Biosciences) were used. All recombinant cytokines were purchased from R&D Systems.
Mouse anti rac1
Manufactured by Abcam
Mouse anti-Rac1 is a primary antibody that specifically binds to the Rac1 protein. Rac1 is a small GTPase that plays a role in regulating the actin cytoskeleton and cell signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti tiam1, by Santa Cruz Biotechnology (1 mentions)
Mouse anti lamin a c, by Cell Signaling Technology (1 mentions)
Mouse anti rorγ, by BD (1 mentions)
Mouse anti gapdh, by Thermo Fisher Scientific (1 mentions)
Irdye 800 goat anti mouse igg, by LI COR (1 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
Irdye 700 goat anti rabbit igg, by LI COR (1 mentions)
Phalloidin 488, by Thermo Fisher Scientific (1 mentions)
Phalloidin 647, by Thermo Fisher Scientific (1 mentions)
Blebbistatin, by Merck Group (1 mentions)
Phalloidin 594, by Thermo Fisher Scientific (1 mentions)
Latrunculin a, by Merck Group (1 mentions)
Eht 1864, by Abcam (1 mentions)
Mouse anti β catenin, by BD (1 mentions)
Rabbit anti β catenin, by Cell Signaling Technology (1 mentions)
Mouse anti claudin 7, by Thermo Fisher Scientific (1 mentions)
3 protocols using mouse anti rac1
1
Antibody-Mediated Protein Interaction Analysis
2
Protein Quantification and Immunoblotting Protocol
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Protein content was estimated by 1D-SDS-PAGE/SYPRO Ruby protein staining-based densitometry, as previously described [22 (link)]. For immunoblotting (10 μg), membranes were probed with primary antibodies [mouse anti-Rac1 (Abcam, 1:1000), mouse anti-Alix mouse (Cell Signalling, 1:1000), mouse anti-TSG101 (Cell Signalling, 1:1000), and mouse anti-GAPDH (Ambion, 1:12,000)] for 1 h in TTBS (50 mM Tris, 150 mM NaCl, 0.05% (v/v) Tween 20) followed by incubation with corresponding secondary antibodies; IRDye 800 goat anti-mouse IgG or IRDye 700 goat anti-rabbit IgG (1:15000, LI-COR Biosciences), for 1 h at RT in TTBS. Immunoblots were imaged using the Odyssey Infrared Imaging System, (v3.0, LI-COR Biosciences, Nebraska USA).
3
Investigating Cell-Cell Adhesion Dynamics
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We used the following primary antibodies: Mouse anti-b-catenin (BD Transduction Cat#610153, 1:100 for IF); rabbit anti-b-catenin (Cell Signaling Cat#8480S, 1:100 for IF); rabbit anti-a-catenin (Sigma Cat#C2081, 1:100 for IF); mouse anti-a-catenin (ENZO Life Sciences Cat#ALX-804-101-C100, 1:100 for IF); mouse anti-P-cadherin (TAKARA Cat#M127, 1:100 for IF); mouse anti-Rac1 (Abcam Cat#ab33186, 1:100 for IF); mouse anticlaudin 7 (Invitrogen Cat#37-4800, 1:100 for IF); rabbit anti-ARPC2 (Abcam Cat#ab133315, 1: 300 for Western blot); rabbit anti-p34-Arc/ARPC2 (EMD Millipore Cat#07-227, 1:100 for For actin staining, we used phalloidin-488 (Invitrogen Cat#A12379, 1:500), phalloidin-594 (Invitrogen Cat#A12381, 1:1000) and phalloidin-647 (Invitrogen Cat#A22287, 1:500). The following inhibitors were used: Blebbistatin (Sigma Cat#B0560); EHT1864 (Abcam Cat#AB229172); CK666 (MERK Cat#182515); and Latrunculin A (Sigma Cat#L5163).
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