Anti parp

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anti-PARP is a lab equipment product that functions as a research tool for the detection and analysis of the Poly(ADP-ribose) Polymerase (PARP) protein. It provides a means to study the role of PARP in various biological processes.

Automatically generated - may contain errors

Lab products found in correlation

Anti β actin, by Merck Group (1 mentions) Tunicamycin, by Thermo Fisher Scientific (1 mentions) Anti flag, by Thermo Fisher Scientific (1 mentions) Anti ub clone p4d1, by Santa Cruz Biotechnology (1 mentions) A23187, by Merck Group (1 mentions) Geldanamycin, by Cayman Chemical (1 mentions) Anti gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) Thapsigargin, by Cayman Chemical (1 mentions) Anti bip, by Thermo Fisher Scientific (1 mentions) Mg132 m 1157, by AG Scientific (1 mentions) Normal mouse igg sc 2025, by Santa Cruz Biotechnology (1 mentions) Anti tubulin dm1a, by Merck Group (1 mentions) Anti flag m2, by Merck Group (1 mentions) Mg 132 cas 133407 82 6, by Merck Group (1 mentions) Cycloheximide c7698 5g, by Merck Group (1 mentions) Anti ha f 7, by Santa Cruz Biotechnology (1 mentions) Protein a g plus agarose sc 2003, by Santa Cruz Biotechnology (1 mentions) Anti myc 9e10, by Santa Cruz Biotechnology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Protease inhibitor cocktail tablet, by Roche (1 mentions)

Spelling variants of this product

Anti-cleaved PARP (4 citations) Anti-PARP (C2-10, (2 citations) Anti-PARP antibody (2 citations)

7 protocols using anti parp

Antibody and Reagent Protocol for ER Stress

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Antibodies for immunoblotting (IB) and immunoprecipitation (IP) used in this study were as follows: anti-β-actin (A1978; Merck), anti-FLAG (PA1-984B; Thermo Fisher Scientific), anti-UBR1 (PA5-42370; Invitrogen), anti-PARP (436400; Invitrogen), anti-inositol-requiring enzyme 1 (IRE1)α (MA5-14991; Invitrogen), anti-pIRE1α Ser724 (PA5-105424; Invitrogen), anti-BiP (PA5-34941; Invitrogen), anti-C/EBP homologous protein (MA1-250; Invitrogen), anti-HA clone 3F10 (12158167001; Merck), anti-His (MA121315; Invitrogen), anti-Ub clone P4D1 (sc-8017; Santa Cruz Biotechnology Inc.), anti-ubiquitin-activating enzyme E1 (PA5-42370; Invitrogen), anti-GFP (sc-9996, Santa Cruz). Secondary antibodies were purchased from Merck. The ER stress-inducing reagents were thapsigargin (#10522; Cayman Chemical), tunicamycin (A61959; Thermo), A23187 (C7522; Sigma), geldanamycin (#13355; Cayman), and brefeldin (b1400; Apexbio Technology). Proteasome inhibitors MG132 (M-1157) and epoxomicin (A2606) were acquired from AG Scientific and ApexBio Technology, respectively.

Le L.T., Park S., Lee J.H., Kim Y.K, & Lee M.J. (2023). N-recognins UBR1 and UBR2 as central ER stress sensors in mammals. Molecules and Cells, 47(1), 100001.

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Immunoblotting Antibody Reagents

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The following antibodies were used: anti-Flag (M2, Sigma-Aldrich, Oakville, AB, Canada), anti-Myc (9E10, Santa Cruz Biotechnology, Dullas, TX, USA), anti-HA (F-7, Santa Cruz Biotechnology, Dullas, TX, USA), anti-tubulin (DM1A, Sigma, Oakville, AB, Canada), anti-PARP (436400, Invitrogen, New York, NY, USA), anti-mouse IgG HRP (85-18-8817-31, ebioscience, San Diego, CA, USA), anti-rabbit IgG HRP (85-18-8816-31, ebioscience, San Diego, CA, USA) and normal mouse IgG (sc-2025, Santa Cruz Biotechnology, Dullas, TX, USA). Protein A/G PLUS Agarose (sc-2003) was purchased from Santa Cruz Biotechnology, Dullas, TX, USA, and Ni-NTA beads (30210) from Qiagen, Duesseldorf, Germany. Cycloheximide (C7698-5G) was purchased from Sigma-Aldrich, Oakville, AB, Canada, and MG132 (CAS 133407-82-6) was from Merck, Darmstadt, Germany.

Ni Y., Tao L., Chen C., Song H., Li Z., Gao Y., Nie J., Piccioni M., Shi G, & Li B. (2015). The Deubiquitinase USP17 Regulates the Stability and Nuclear Function of IL-33. International Journal of Molecular Sciences, 16(11), 27956-27966.

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Western Blot Analysis of O-GlcNAc Signaling

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HCT116 and HepG2 cell samples were lysed with NP40 assay buffer containing 1× PBS, 1% NP40, 1 mM EDTA, and protease inhibitor cocktail tablets (Roche, Mannheim, Germany). Proteins were separated on 8–15% SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare Life science, Boston, MA, USA). The membranes were blocked with 5% skim milk at room temperature for 1 h and then incubated overnight with primary antibodies at 4 °C. Anti-O-GlcNAc was purchased from Sigma-Aldrich (St Louis, MO, USA). Anti-PARP was purchased from Invitrogen (Waltham, MA, USA). Anti-total caspase-3, cleaved caspase-3, IRE1α, p-p65, p65, p-IKKα/β, IKKα/β, p-IκB and p-eIF2α were purchased from Cell Signaling Technology. Anti-OGT, PERK, Bcl2, Bax, cytochrom c and β-actin were purchased from Santa Cruz. Membranes were washed with TBST and incubated with antimouse or antirabbit horseradish peroxidase (HRP)-conjugated IgG secondary antibody for 30 min. The signal was visualized using an ECL Plus Detection System (GE Healthcare Life Sciences) and the bands were quantified using ImageJ software (NIH, United States).

Lee S.J., Lee D.E., Choi S.Y, & Kwon O.S. (2021). OSMI-1 Enhances TRAIL-Induced Apoptosis through ER Stress and NF-κB Signaling in Colon Cancer Cells. International Journal of Molecular Sciences, 22(20), 11073.

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Monascin-Induced Apoptosis Signaling

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Monascin (cat. no. 52442) and other chemicals and reagents (unless otherwise stated) were purchased from Sigma-Aldrich; Merck KGaA. Media, FBS (cat. no. 26140079) and culture supplements were purchased from Invitrogen; Thermo Fisher Scientific, Inc. Anti-PARP (cat. no. 9542), caspase-3 (cat. no. 9665), E-cadherin (cat. no. 3195), β-catenin (cat. no. 9562) and β-actin (cat. no. 4970) were purchased from Cell Signaling Technology, Inc.

Chen K.Y., Lin J.A., Yao H.Y., Hsu A.C., Tai Y.T, & Ho B.Y. (2020). Monascin accelerates anoikis in circulating tumor cells and prevents breast cancer metastasis. Oncology Letters, 20(5), 166.

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Osimertinib and FAK Inhibitor Signaling

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Osimertinib was purchased from Selleckchem (S7297), and FAK inhibitor 14 and FAK autophosphorylation inhibitor from Abcam (ab144503). The following antibodies were obtained from Cell Signaling Technology: anti-FAK (#3285), anti-Phos-FAK (Tyr397) (#3283), anti-paxillin (#2542), anti-Phos-paxillin (Tyr118) (#69363), anti-thrombospondin-1 (D7E5F) (#37879), anti-AKT3 (#4059), anti-COL5A1(#37304), anti-E-cadherin (#3195), anti-PARP (#9542), anti-caspase-3 (#9662), anti-cleaved PARP (Asp214) (#5625), anti-N-cadherin (D4R1H) (#13116), anti-E-cadherin (24E10) (#3195), anti-vimentin (D21H3) (#5741), anti-rabbit IgG HRP-linked (#7074P2), and anti-mouse IgG HRP-linked (#7076P2).
The anti-CADM1 (PA3-16744) and anti-BICC (PA5-116342) were purchased from Thermo Fisher. The anti-IGFBP7 (ab74169) and anti-PTPRM (ab231607) were purchased from Abcam. The anti-β-actin (A2228) and anti-RAB32 (HPA025731) were purchased from Sigma-Aldrich.

Kosibaty Z., Brustugun O.T., Zwicky Eide I.J., Tsakonas G., Grundberg O., De Petris L., McGowan M., Hydbring P, & Ekman S. (2022). Ras-Related Protein Rab-32 and Thrombospondin 1 Confer Resistance to the EGFR Tyrosine Kinase Inhibitor Osimertinib by Activating Focal Adhesion Kinase in Non-Small Cell Lung Cancer. Cancers, 14(14), 3430.

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Protein Expression Analysis via Western Blot

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Proteins were resolved using 4–12% polyacrylamide gel electrophoresis Bis-Tris gels (Life Technology, Carlsbad, CA) and electrotransferred to nitrocellulose membranes. After blocking in Tris-buffered saline (TBS) 0.1%, Tween 20%, and 5% bovine serum albumin, membranes were immunostained overnight with appropriate primary antibodies followed by incubation with secondary antibodies conjugated to HRP. Immunoreactive bands were visualized by enhanced chemiluminescence (ECL Supersignal West Pico; Thermo Fisher Scientific) with a Syngene camera. Quantification of chemiluminescent signals was done with the GeneTools software v4.3.8.0 from Syngene. List of antibodies used in this work: anti-CASPASE-3 (CST, Cat#9662; 1/1000), Anti-ACTIN (Millipore, Cat# MAB1501; 1/10,000), anti-CALCRL (Elabscience, Cat# ESAP13421; 1/1000), anti-RAD51 (Abcam, Cat# ab133534; 1/1000), anti-BCL2 (CST, Cat# 2872; 1/1000), anti-E2F1 (C-20)(Santa Cruz, Cat# sc-193; 1/1000), anti-CHK1 (Santa Cruz, Cat# sc-8408; 1/1000), anti-RAMP1 (3B9) (Santa Cruz, Cat# sc-293438; 1/1000), anti-RAMP2 (B-5) (Santa Cruz, Cat# sc-365240; 1/1000), anti-RAMP3 (G-1) (Santa Cruz, Cat# sc-365313; 1/1000), anti-ADM (Thermo Fisher Scientific, Cat# PA5-24927; 1/1000), anti-CGRP (Abcam, Cat# ab47027; 1/1000), anti-PARP (Thermo Fisher Scientific, Cat# 44-698G; 1/1000), and anti-alpha/beta-Tubulin (CST, Cat# 2148; 1/1000).

Larrue C., Guiraud N., Mouchel P.L., Dubois M., Farge T., Gotanègre M., Bosc C., Saland E., Nicolau-Travers M.L., Sabatier M., Serhan N., Sahal A., Boet E., Mouche S., Heydt Q., Aroua N., Stuani L., Kaoma T., Angenendt L., Mikesch J.H., Schliemann C., Vergez F., Tamburini J., Récher C, & Sarry J.E. (2021). Adrenomedullin-CALCRL axis controls relapse-initiating drug tolerant acute myeloid leukemia cells. Nature Communications, 12, 422.

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Protein Expression Analysis by Immunoblotting

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Cell lysate was prepared to perform SDS-polyacrylamide gel electrophoresis and immunoblot analysis [24] . The protein expression was detected using anti-LMP1, anti-caspase, antiphospho-ERK, anti-ERK, anti-IκBα, anti-phospho-IκBα and anti-PARP antibodies, which were purchased from Thermo Scientific (CA, USA) and Cell signaling (Danvers, MA), respectively. The band intensities were scanning densitometry-quantitated.

Lin T. (2021). "The Ethyl Acetate Subfraction of Polygonum Cuspidatum Root Containing Emodin Induce EBV- Positive Cells Apoptosis". Biomedical Journal of Scientific & Technical Research, 35(5).

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