Wild-type (WT) C57BL/6 mice were purchased from Koatech (Pyeongtaek, Korea). Fat-1 transgenic mice were kindly provided by Dr. Jing X. Kang (Department of Medicine, Massachusetts General Hospital and Harvard Medical School, USA). Murine bone marrow-derived macrophages (BMDMs) were differentiated for 5–7 days in medium containing macrophage colony-stimulating factor, as described previously [22 (link)]. The human retinal pigment epithelial cell line ARPE-19 was purchased from the American Type Culture Collection (Manassas, VA, USA) and cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco BRL, Grand Island, NY, USA) or with nutrient mixture F-12, 10% FBS, and 1% antibiotic-antimycotic (Anti-Anti, Gibco BRL). ARPE-19 cells were passaged using 0.25% trypsin-EDTA (Life Technologies, Carlsbad, CA, USA) every 2–3 days.
Wt c57bl 6 mice
Manufactured by Koatech
WT C57BL/6 mice are a widely used inbred strain of laboratory mice. They are genetically homogeneous, allowing for consistent and reproducible research results. These mice serve as a common control strain for various biomedical studies.
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5 protocols using wt c57bl 6 mice
1
Murine Macrophage and ARPE-19 Cell Culture
2
Generation of Tet2 Deficient Mice
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WT C57BL/6 mice were purchased from Koatech (Pyeongtaek, Gyeongi-do, Korea). Floxed Tet2 transgenic (B6;129S-Tet2tm1.1Iaai/J, Tet2fl/fl) mice were obtained from The Jackson Laboratory (Bar Harbor, ME). To generate Tet2 deficient mice (referred to as Tet2−/− mice), CD4-Cre transgenic mice were crossed to floxed Tet2 transgenic mice. All animal experimentations were conducted in accordance with guidelines and approval of the International Animal Care and Use Committees of Hallym University (Hallym 2015-63).
3
Generating G2A Knockout Mice
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We purchased WT C57BL/6 mice from Koatech (Pyeongtaek, Korea). The G2A knockout (KO) mice (36 (link)) on the C57BL/6 background, as we described previously, we received from Dr. DK Song (Hallym University). We did all animal experiments in accordance with guidelines and approval of the International Animal Care and Use Committees of Hallym University (Hallym 2018-9, 2019-18).
4
C57BL/6 Mice and P2Y2R Knockout
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WT C57BL/6 mice (6-week old) were purchased from Koatech Co. (Pyeongtaek, South Korea) and homozygous P2Y2R KO mice on C57BL/6 background (strain B6.129P2-P2ry2tm1Bhk/J) were obtained from Jackson Laboratory. All mice were maintained in the animal facility at Gyeongsang National University (GNU-171130-M0058). All animal experiments were approved by the Institutional Board of Animal Research at Gyeongsang National University, and performed according to the National Institutes of Health guidelines for laboratory animal care. Mice were housed with an alternating 12-h light/dark cycle and provided with water and standard chow ad libitum.
5
C57BL/6 Mice Maintenance Protocol
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Wild type (WT) C57BL/6 mice were purchased from Koatech Co. (Pyeongtaek, Republic of Korea) and were maintained in the animal facility at Gyeongsang National University. All animal experiments were approved by the Institutional Board of Animal Research at Gyeongsang National University and performed according to the National Institutes of Health guidelines for laboratory animal care (GNU-200612-M0036; 29 June 2020). Mice were housed with an alternating 12 h light/dark cycle and provided freely with water and standard chow.
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