Anti cdk2 and anti cdk4

The materials and their manufacturers’ information used in this study are the following: Dulbecco’s modified Eagle medium (DMEM)/F12, Penicillin-Streptomycin (P/S), 0.25% trypsin-EDTA and 10% Fetal Bovine Serum (FBS) from Gibco BRL (Grand Island, NY, USA); dimethyl sulfoxide from Invitrogen (Carlsbad, CA, USA); Tween^® 20 and ECLTM Western blotting detection reagent from Amersham Life Science (Arlington Heights, IL, USA); etoposide from Selleckchem.com (Houston, TX, USA); anti-β Actin from Sigma (St. Louis, MO, USA); anti-Phospho-Histone H3 and anti-Histone H3 from Cell Signaling Technology (MA, USA); anti-CDK2 and anti-CDK4 from Santa Cruz Biotechnology; anti-GFAP from EMD Millipore (MA, USA); senescence detection kit from Abcam; Agilent Seahorse XF Cell Mito Test Kit from Agilent Technology (CA, USA); Doxorubicin from Sigma; Alexa Fluor^® 594 conjugated Escherichia coli (K-12 strain) BioParticles^® from Thermo Fisher Scientific (MA, USA); Tetramethylrhodamine Methyl Ester (TMRM) from Thermo Fisher Scientific.

The following materials were used in this study: Dulbecco’s modified Eagle medium (DMEM)/F12, Penicillin-Streptomycin (P/S), 0.25% trypsin-EDTA from Gibco BRL (Grand Island, NY, USA); 10% Fetal Bovine Serum (FBS) from Gibco BRL, and dimethyl sulfoxide from Invitrogen (Carlsbad, CA, USA); Tween^® 20 and ECL^TM Western blotting detection reagent from Amersham Life Science (Arlington Heights, IL, USA); tenovin-1 from Tocris bioscience (Minneapolis, MN, USA); anti-β-actin from Sigma (St. Louis, MO, USA); anti-iNOS from Abcam (Cambridgeshire, UK); anti-COX2 from Santa Cruz Biotechnology (CA, USA); anti-phospho-H3 and anti-histone H3 from Cell Signaling Technology (MA, USA); anti-CDK2 and anti-CDK4 from Santa Cruz Biotechnology; anti-GFAP from EMD Millipore (MA, USA); senescence detection kit from Abcam.