Quanta bio studio

Manufactured by Thermo Fisher Scientific

The Quanta bio-studio is a versatile laboratory instrument designed for a range of applications. It is capable of performing high-throughput analysis and quantification of various biomolecules, including proteins, nucleic acids, and small molecules. The core function of the Quanta bio-studio is to provide accurate and reliable data to support scientific research and discovery.

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Lab products found in correlation

Rna extraction kit, by Qiagen (2 mentions) Qscript kit, by Quanta Biosciences (1 mentions) Taqman mirna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Fast sybr green master mix, by Thermo Fisher Scientific (1 mentions) Qscript cdna supermix, by Quanta Biosciences (1 mentions)

2 protocols using quanta bio studio

Quantitative RT-PCR for miRNA and mRNA

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Total RNA was extracted from cells or tissues using a RNA extraction kit (Qiagen) according to the manufacturers’ instructions. RNA was reverse transcribed to cDNA using Q-script kit (Quanta Biosciences, Gaithersburg, MD) and TaqMan MiRNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA) according to manufacturer’s instructions. Quantitative real-time PCR was performed in a Quanta bio-studio (Applied Biosystems, Grand Island, NY) using the Taqman–based method for detection of gene amplification according to the manufacturer’s instructions. Relative miRNA or mRNA expression of target gene was normalized to the U6 control or GAPDH gene, respectively. Data was represented as fold change versus respective control.

Suresh Babu S., Thandavarayan R.A., Joladarashi D., Jeyabal P., Krishnamurthy S., Bhimaraj A., Youker K.A, & Krishnamurthy P. (2016). MicroRNA-126 overexpression rescues diabetes-induced impairment in efferocytosis of apoptotic cardiomyocytes. Scientific Reports, 6, 36207.

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Quantitative RT-PCR for Gene Expression Analysis

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Total cellular and exosome RNA were extracted using a RNA extraction kit (Cat# 217004, Qiagen), and RNA was reverse transcribed to cDNA using qScript cDNA SuperMix (Cat# 95048–025, Quanta Biosciences) according to the manufacturer’s instructions. Quantitative RT-PCR was performed using Quanta bio-studio (Applied Biosystems, Grand Island, NY) using the Fast SYBR Green Master Mix (Cat# 4385612, Applied Biosystems) for detection of gene amplification by following manufacturer’s instructions. Relative mRNA expression of target gene was normalized to the β-Actin or GAPDH gene. The data were represented as fold change versus respective control.

Govindappa P.K., Patil M., Garikipati V.N., Verma S.K., Saheera S., Narasimhan G., Zhu W., Kishore R., Zhang J, & Krishnamurthy P. (2019). Targeting exosome-associated human antigen R attenuates fibrosis and inflammation in diabetic heart. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(2), 2238-2251.

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