Nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) and data analysis were a as described39 . Briefly, samples containing MNase-digested chromatin were size-fractionated by SDS-PAGE, gels were cut in 2-mm slices, and subjected to dithiotreitol-reduction, iodoacetamide-alkylation and trypsin-digestion. LC-MS/MS was performed on an 1100 series capillary liquid chromatography system (Agilent Technologies) coupled to an LTQ-Orbitrap XL mass spectrometer (Thermo Scientific) operating in positive mode. Raw MS data were analyzed using the MaxQuant software. A false discovery rate of 0.01 for proteins and peptides and a minimum peptide length of 6 amino acids were set. The Andromeda search engine was used to search MS/MS spectra against the International Protein Index (IPI) human database. Statistical analysis was performed with Perseus (1.5.0.30)17 (link).
1100 series capillary liquid chromatography system
Manufactured by Agilent Technologies
The Agilent 1100 series capillary liquid chromatography system is a compact, high-performance liquid chromatography (LC) instrument designed for analytical-scale separations. It features a capillary-flow configuration, allowing for the use of small sample and mobile phase volumes. The system is capable of performing sensitive and precise chromatographic analyses across a wide range of applications.
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Lab products found in correlation
2 protocols using 1100 series capillary liquid chromatography system
1
Chromatin Proteome Profiling by Nanoflow LC-MS/MS
2
Chromatin Proteome Profiling by Nanoflow LC-MS/MS
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Nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) and data analysis were a as described39 . Briefly, samples containing MNase-digested chromatin were size-fractionated by SDS-PAGE, gels were cut in 2-mm slices, and subjected to dithiotreitol-reduction, iodoacetamide-alkylation and trypsin-digestion. LC-MS/MS was performed on an 1100 series capillary liquid chromatography system (Agilent Technologies) coupled to an LTQ-Orbitrap XL mass spectrometer (Thermo Scientific) operating in positive mode. Raw MS data were analyzed using the MaxQuant software. A false discovery rate of 0.01 for proteins and peptides and a minimum peptide length of 6 amino acids were set. The Andromeda search engine was used to search MS/MS spectra against the International Protein Index (IPI) human database. Statistical analysis was performed with Perseus (1.5.0.30)17 (link).
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