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Dab bond polymer refine

Manufactured by Leica
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The DAB-Bond Polymer Refine is a laboratory equipment product designed for use in immunohistochemistry and in situ hybridization workflows. It is a polymer-based detection system that enhances the visualization of target molecules in tissue samples. The core function of this product is to provide a sensitive and reliable means of amplifying and detecting specific biological targets within the tested samples.

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Lab products found in correlation

Bond max automated immunostainer, by Leica (2 mentions) Rabbit anti guinea pig, by Abcam (2 mentions) Goat anti rabbit poly hrp, by Leica (2 mentions) Calf thymus dsdna, by Merck Group (2 mentions) Collagenase type 4, by Worthington (2 mentions)

2 protocols using dab bond polymer refine

1

Autoantibody and Insulin Detection Assays

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine serum was tested by ELISA for reactivity to calf thymus dsDNA (Sigma-Aldrich), and sm-RNP (ATR1-10; Arotech Diagnostics Limited) which has previously been described58 (link).
To obtain RNA samples for quantitative PCR, murine spleens were digested with collagenase Type 4 (Worthington Biochemical), followed by calcium chelation, RBC lysis and made into single cell suspensions.
Insulin was detected by immunohistochemistry in paraffin embedded pancreas tissue sections using a Leica Bond MAX Automated Immunostainer (Leica Microsystems) and guinea pig anti-insulin (Dako-A0564) primary antibody with rabbit anti-guinea pig (Abcam) and goat anti-rabbit poly-HRP (Leica Microsystems) secondary antibodies. Visualization was with DAB-Bond Polymer Refine (Leica Microsystems).
Gorman J.A., Hundhausen C., Errett J.S., Stone A.E., Allenspach E.J., Ge Y., Arkatkar T., Clough C., Dai X., Khim S., Pestal K., Liggitt D., Cerosaletti K., Stetson D.B., James R.G., Oukka M., Concannon P., Gale M J.r., Buckner J.H, & Rawlings D.J. (2017). The A946T variant IFIH1 RNA sensor mediates an interferon program that limits viral infection but increases the risk for autoimmunity. Nature immunology, 18(7), 744-752.
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2

Autoantibody and Insulin Detection Assays

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine serum was tested by ELISA for reactivity to calf thymus dsDNA (Sigma-Aldrich), and sm-RNP (ATR1-10; Arotech Diagnostics Limited) which has previously been described58 (link).
To obtain RNA samples for quantitative PCR, murine spleens were digested with collagenase Type 4 (Worthington Biochemical), followed by calcium chelation, RBC lysis and made into single cell suspensions.
Insulin was detected by immunohistochemistry in paraffin embedded pancreas tissue sections using a Leica Bond MAX Automated Immunostainer (Leica Microsystems) and guinea pig anti-insulin (Dako-A0564) primary antibody with rabbit anti-guinea pig (Abcam) and goat anti-rabbit poly-HRP (Leica Microsystems) secondary antibodies. Visualization was with DAB-Bond Polymer Refine (Leica Microsystems).
Gorman J.A., Hundhausen C., Errett J.S., Stone A.E., Allenspach E.J., Ge Y., Arkatkar T., Clough C., Dai X., Khim S., Pestal K., Liggitt D., Cerosaletti K., Stetson D.B., James R.G., Oukka M., Concannon P., Gale M J.r., Buckner J.H, & Rawlings D.J. (2017). The A946T variant IFIH1 RNA sensor mediates an interferon program that limits viral infection but increases the risk for autoimmunity. Nature immunology, 18(7), 744-752.
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