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Nod scid il2rgnull3tg hscf hgm csf hil3

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The NOD-scid IL2Rgnull3Tg (hSCF/hGM-CSF/hIL3) is a transgenic mouse line that expresses human stem cell factor (hSCF), human granulocyte-macrophage colony-stimulating factor (hGM-CSF), and human interleukin-3 (hIL3). This mouse line is designed to support the engraftment and development of human hematopoietic cells.

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Edta tube, by Greiner (2 mentions)

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NOD/SCID

3 protocols using nod scid il2rgnull3tg hscf hgm csf hil3

1

Xenograft Model for Leukemia Treatment

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All animal experiments were conducted in accordance to guidelines approved by the IACUC at Vanderbilt University Medical Center. Male NSGS [NOD-scid IL2Rgnull3Tg (hSCF/hGM-CSF/hIL3)] mice (The Jackson Laboratory), 6 – 8 weeks old were irradiated with 100 cGy microwave radiation. Twenty-four hours later, mice were transplanted with 1 × 106 MV-4–11 cells via tail vein injections in each irradiated mouse. Mice were randomized post xenograft transplantation into cages of 5. Prior to treatment, peripheral microchimerism was documented at week 1. Upon establishing microchimerism, mice were treated with either 0.1 mg/kg or 0.03 mg/kg ammocidin in saline or saline vehicle i.p. for 5 days on, 2 days off for 2 weeks. Murine CBC was analyzed from blood collected into EDTA tubes (Greiner Bio-One) and analyzed with a Hemavet (Drew Scientific) analysis system.
Reisman B.J., Guo H., Ramsey H.E., Wright M.T., Reinfeld B.I., Ferrell P.B., Sulikowski G.A., Rathmell W.K., Savona M.R., Plate L., Rubinstein J.L, & Bachmann B.O. (2021). Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase. Nature chemical biology, 18(4), 360-367.
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2

Engrafting Primary AML Cells in NSGS Mice

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2×106 primary AML mononuclear cells were engrafted in 7-9- week-old female NSGS (NOD-scid IL2Rgnull3Tg(hSCF/hGMCSF/ hIL3)) mice (The Jackson Laboratory) as described in the Online Supplementary Materials and Methods. Chimerism was assessed weekly in the peripheral blood, and, at time of tissue harvest, in the bone marrow and spleen. Animal experiments were conducted in accordance with guidelines approved by the IACUC at VUMC.
Cojocari D., Smith B.N., Purkal J.J., Arrate M.P., Huska J.D., Xiao Y., Gorska A., Hogdal L.J., Ramsey H.E., Boghaert E.R., Phillips D.C, & Savona M.R. (2021). Pevonedistat and azacitidine upregulate NOXA (PMAIP1) to increase sensitivity to venetoclax in preclinical models of acute myeloid leukemia. Haematologica, 107(4), 825-835.
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3

Xenograft Model for Leukemia Treatment

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All animal experiments were conducted in accordance to guidelines approved by the IACUC at Vanderbilt University Medical Center. Male NSGS [NOD-scid IL2Rgnull3Tg (hSCF/hGM-CSF/hIL3)] mice (The Jackson Laboratory), 6 – 8 weeks old were irradiated with 100 cGy microwave radiation. Twenty-four hours later, mice were transplanted with 1 × 106 MV-4–11 cells via tail vein injections in each irradiated mouse. Mice were randomized post xenograft transplantation into cages of 5. Prior to treatment, peripheral microchimerism was documented at week 1. Upon establishing microchimerism, mice were treated with either 0.1 mg/kg or 0.03 mg/kg ammocidin in saline or saline vehicle i.p. for 5 days on, 2 days off for 2 weeks. Murine CBC was analyzed from blood collected into EDTA tubes (Greiner Bio-One) and analyzed with a Hemavet (Drew Scientific) analysis system.
Reisman B.J., Guo H., Ramsey H.E., Wright M.T., Reinfeld B.I., Ferrell P.B., Sulikowski G.A., Rathmell W.K., Savona M.R., Plate L., Rubinstein J.L, & Bachmann B.O. (2021). Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase. Nature chemical biology, 18(4), 360-367.
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