When the tumor size was greater than 200 mm3, the B-MN, MN-I, and MN-pB/I patches were inserted into tumor sites of the mice bearing A375 tumors. A Xenogen IVIS Spectrum imaging system (Perkin Elmer, USA) was used to measure the in vivo fluorescence intensity of ICG (Ex: 735 nm/Em: 805 nm) 0.5 h following insertion of the patches. Several mice were sacrificed for ex vivo imaging of the major organs (hearts, livers, spleens, lungs, and kidneys) and tumor tissues. For the PAI, photoacoustic (PA) signals of tumor-bearing mice were monitored by a VEVO LASER PAI system (VEVO 2100, FUJIFILM Visual Sonics, INC, USA).
Vevo laser pai system vevo 2100
Manufactured by Fujifilm
Sourced in United States
The VEVO LASER PAI system (VEVO 2100) is a high-resolution photoacoustic imaging platform designed for preclinical research. It utilizes laser-induced photoacoustic signals to generate high-contrast, high-resolution images of biological tissues.
Automatically generated - may contain errors
3 protocols using vevo laser pai system vevo 2100
1
Fluorescence and Photoacoustic Imaging of Tumor Microenvironment
2
ICG-Based Multimodal Imaging Protocol
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In vitro FI was performed using a Xenogen IVIS Spectrum imaging system (Perkin Elmer, USA) at 745 nm excitation wavelength using an 820 nm filter. The samples were diluted according to the concentration of ICG from 1.0 to 200 µg/mL. PAI experiments were performed using a VEVO LASER PAI system (VEVO 2100, FUJIFILM Visual Sonics, INC, USA). The Lipo/pB-DOX/ICG liposomes were diluted to a series of ICG concentrations from 0 to 200 µg/mL and added to a centrifuge tube for PAI.
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In vitro FI was performed using a Xenogen IVIS Spectrum imaging system (Perkin Elmer, USA) at 745 nm excitation wavelength using an 820 nm filter. The samples were diluted according to the concentration of ICG from 1.0 to 200 µg/mL. PAI experiments were performed using a VEVO LASER PAI system (VEVO 2100, FUJIFILM Visual Sonics, INC, USA). The Lipo/pB-DOX/ICG liposomes were diluted to a series of ICG concentrations from 0 to 200 µg/mL and added to a centrifuge tube for PAI.
3
Photoacoustic Imaging of Tumor-Bearing Mice
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First, tumor-bearing mice with a volume of about 200 mm3 were randomly selected. Experimental group mice were injected with 200 μL of PDA@Lipo/DOX/ICG nanoparticle solution via tail vein, and the control group mice were injected with an equal amount of free ICG as a control. After 1 h and 24 h administration, respectively, the mice were anesthetized with isoflurane and then placed in IVIS Spectrum imaging system (Perkin Elmer, USA) for comparison and observation (Ex: 745 nm, Em: 820 nm). Finally, the mice were sacrificed, and their tumor tissues and main organs (heart, liver, spleen, lung, and kidney) were obtained for the ex vivo imaging. For the PAI, photoacoustic signals of tumor-bearing mice were monitored using a VEVO LASER PAI system (VEVO 2100, FUJIFILM Visual Sonics, INC, USA) at different time points.
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