Anti mouse mhcii clone m5 114

Manufactured by BioLegend

Sourced in United States

Anti-mouse MHCII (clone M5/114.15.2) is a lab equipment product that can be used to detect major histocompatibility complex class II (MHCII) molecules on mouse cells. This clone is specific for the I-A/I-E MHCII isotypes.

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Lab products found in correlation

Anti mouse human cd11b clone m1 70, by BioLegend (5 mentions) Anti mouse f4 80 clone bm8, by BioLegend (4 mentions) Lsrfortessa cell analyzer, by BD (3 mentions) Anti mouse cd206 clone c068c2, by BioLegend (3 mentions) Anti mouse cd45 clone 30 f11, by BioLegend (3 mentions) Antimouse gr1 clone rb6 8c5, by BioLegend (2 mentions) 7 aminoactinomycin d 7 aad, by Thermo Fisher Scientific (2 mentions) Annexin 5, by BD (2 mentions) Anti human cd47 clone b6h12, by BD (2 mentions) Facscanto 2, by BD (2 mentions) Anti mouse ly6c clone hk1, by BioLegend (2 mentions) Anti mouse ifn γ xmg1, by Thermo Fisher Scientific (1 mentions) Anti mouse cd45 clone 30 f11, by BD (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Rabbit anti ifngr1, by Merck Group (1 mentions) Anti mouse cd3 clone 17a2, by BioLegend (1 mentions) Anti mouse b220 clone ra3 6b2, by BioLegend (1 mentions) Anti mouse cd11b m1 70, by Thermo Fisher Scientific (1 mentions) Anti mouse cd4 clone gk1, by BD (1 mentions) Anti human cd3 sk7, by BioLegend (1 mentions)

Spelling variants of this product

MHCII (89 citations) M5/114.15.2 (24 citations) MHCII (M5/114.15.2, (19 citations) Anti-MHCII (19 citations) MHCII (clone M5/114.15.2, (16 citations) Clone M5/114.15.2 (6 citations) Anti-MHCII (clone M5/114.15.2, (4 citations)

6 protocols using anti mouse mhcii clone m5 114

Immunoblotting and Flow Cytometry Protocols

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For immunoblotting, these antibodies are used: rabbit anti-STAT1 (CST, #14994), rabbit anti–phospho-STAT1 (CST, #7649), rabbit anti-EZH2 (CST, #5246), mouse anti–MHC-I class I (Santa Cruz Biotechnology, #sc-55582), rabbit anti-IFNGR1 (Millipore, #MABF753), and mouse anti–β-actin (Sigma-Aldrich, #A5441).
For flow cytometry, the following fluorochrome-conjugated antibodies are used: anti-mouse H-2K^b/H-2D^b (clone 28-8-6, BioLegend), anti-mouse H-2Kb bound to SIINFEKL (clone 25-D1.16, BioLegend), anti-mouse CD3 (clone 17A2, BioLegend), anti-mouse CD28 (clone 37.51, BioLegend), anti-mouse granzyme B (clone GB11, BioLegend), anti-mouse B220 (clone RA3-6B2, BioLegend), anti-mouse CD49b (clone DX5, BioLegend), anti-mouse Gr-1 (clone RB6-8C5, BioLegend), anti-mouse MHC-II (clone M5/114.15.2, BioLegend), anti-mouse F4/80 (clone BM8, BioLegend), anti-mouse IFN-γ (XMG1.2, eBioscience), anti-mouse CD11b (M1/70, eBioscience), anti-mouse NK1.1 (clone PK136, BD), anti-mouse CD103 (clone M290, BD), anti-mouse CD206 (clone MR5D3, BD), anti-mouse CD24 (clone M1/69, BD), anti-mouse CD4 (clone GK1.5, BD), anti-mouse CD45 (clone 30-F11, BD), anti-mouse CD8a (clone 53-6.7, BD), anti-mouse Foxp3 (clone MF23, BD), anti-mouse γδ TCR (clone GL3, BD), anti-human CD3 (SK7, BioLegend), anti-human CD4 (SK3, BioLegend), anti-human CD8 (SK1, BioLegend), and anti-human 137(4-1BB) (4B4-1, BioLegend).

Guo W., Wang Y., Yang M., Wang Z., Wang Y., Chaurasia S., Wu Z., Zhang M., Yadav G.S., Rathod S., Concha-Benavente F., Fernandez C., Li S., Xie W., Ferris R.L., Kammula U.S., Lu B, & Yang D. (2021). LincRNA-immunity landscape analysis identifies EPIC1 as a regulator of tumor immune evasion and immunotherapy resistance. Science Advances, 7(7), eabb3555.

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Multiparameter Flow Cytometry Analysis

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Anti-mouse CD47 (clone miap301; BioLegend), anti-human CD47 (clone B6H12; BD Biosciences), anti-mouse F4/80 (clone BM8; BioLegend), anti-mouse/human CD11b (clone M1/70; BioLegend), anti-mouse MHC-II (clone M5/114.15.2; BioLegend), anti-mouse CD206 (clone C068C2; BioLegend), anti-mouse Sirpα (clone P84; BioLegend) and anti-human CD206 (clone 15-2; BioLegend) were used for FACS analyses. Antibodies were phycoerythrin (PE), PE Cy7, APC, APC Cy7, Alexa Fluor 700, BV787, or BV605 conjugated, or fluorophore-conjugated secondary antibodies were used. Sytox blue was used to exclude dead cells. Annexin V (BD Biosciences) and 7-aminoactinomycin D (7-AAD; ThermoFisher) were used to evaluate cell viability of macrophages on cabazitaxel treatment. Flow cytometry was performed using the BD LSRFortessa cell analyzers.

Cao X., Li B., Chen J., Dang J., Chen S., Gunes E.G., Xu B., Tian L., Muend S., Raoof M., Querfeld C., Yu J., Rosen S.T., Wang Y, & Feng M. (2021). Effect of cabazitaxel on macrophages improves CD47-targeted immunotherapy for triple-negative breast cancer. Journal for Immunotherapy of Cancer, 9(3), e002022.

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Multiparametric Flow Cytometry Analysis

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The following antibodies were used for flow cytometry analysis (dilutions are indicated): anti-mouse CD45 (clone 30-F11, 1:100), anti-mouse/human CD11b (clone M1/70, 1:300), anti-mouse Ly6C (clone HK1.4, 1:300), anti-mouse MHCII (clone M5/114.15.2, 1:200), anti-mouse CD64 (clone X54-5/7.1, 1:50), anti-mouse Ly6G (clone 1A8, 1:100), and anti-mouse Tim4 (clone RMT4-54, 1:50), which were purchased from BioLegend, San Diego, CA, USA. Anti-mouse F4/80 (clone A3-1, 1:50) was purchased from BIORAD. The staining for ROS was performed with 0.1 mM of 2,7-dichlorodihydrofluoresceindiacetate (Molecular Probes Invitrogen). Staining for apoptosis and necrosis markers with Annexin V and propidium iodide was performed with MEBCYTO-Apoptosis Kit (MBL International Corporation). Cells were analyzed with BD FACSCanto™ II (BD Bioscience). Flow cytometry analysis was performed using FlowJo software (TreeStar, Ashland, OR, USA).

Graubardt N., Vugman M., Mouhadeb O., Caliari G., Pasmanik-Chor M., Reuveni D., Zigmond E., Brazowski E., David E., Chappell-Maor L., Jung S, & Varol C. (2017). Ly6Chi Monocytes and Their Macrophage Descendants Regulate Neutrophil Function and Clearance in Acetaminophen-Induced Liver Injury. Frontiers in Immunology, 8, 626.

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Phenotyping Liver Non-Parenchymal Cells

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Non-parenchymal liver cells were incubated with monoclonal antibody 2·4G2 for FcR blocking (BioLegend, San Diego, CA, USA) and then exposed at 4°C to a mixture of the following antibodies (dilutions are indicated):anti-mouse CD45 (clone 30-F11, 1:100), anti-mouse/human CD11b (clone M1/70, 1:300), anti-mouse Ly6C (clone HK1.4, 1:300), anti-mouse MHCII (clone M5/114.15.2, 1:200), anti-mouse CD11c (clone N418, 1:100), anti-mouse CD3ϵ (clone 145-2c11, 1:100), anti-mouse CD8a (clone 53-6.7, 1:100), anti-mouse CD4 (clone GK1.5, 1:100), anti-mouse TCRβ (clone 457-597, 1:100) all were purchased from BioLegend, San Diego, CA. Anti-mouse F4/80 (clone REA 126, 1:100) and anti-mouse Tim4 (clone REA999, 1:100) were purchased from Miltenyi Biotech.
For IL-17A staining, cells were first stained for surface markers, then the cells were fixed and permeabilized prior to intra-cellular staining with IL-17 (Clone TC11-18H10.1, 1:50, BioLegend). Cells were analyzed with BD FACS Canto™ II (BD Bioscience) or sorted with a FACSAria flow cytometer (BD Bioscience). Flow cytometry analysis was performed using FlowJo software (TreeStar, Ashland, OR).

Reuveni D., Brezis M.R., Brazowski E., Vinestock P., Leung P.S., Thakker P., Gershwin M.E, & Zigmond E. (2021). Interleukin 23 Produced by Hepatic Monocyte-Derived Macrophages Is Essential for the Development of Murine Primary Biliary Cholangitis. Frontiers in Immunology, 12, 718841.

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Multiparametric Flow Cytometry Analyses

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Anti-mouse F4/80 (clone BM8, BioLegend), anti-mouse/human CD11b (clone M1/70, BioLegend), anti-mouse MHCII (clone M5/114.15.2, BioLegend), anti-mouse iNOS (clone W16030C, BioLegend), anti-mouse CD206 (clone C068C2, BioLegend), and anti-mouse CSF1R (clone AFS98, BioLegend) were used for FACS analyses. The antibodies were PE-, PE-Cy7–, allophycocyanin (APC)–, APC Cy7–, or Alexa Fluor 700–conjugated, or fluorophore-conjugated secondary antibodies were used. Sytox blue or Zombie Violet was used to exclude dead cells. Flow cytometry was performed using the BD LSRFortessa cell analyzers.

Cao X., Chen J., Li B., Dang J., Zhang W., Zhong X., Wang C., Raoof M., Sun Z., Yu J., Fakih M.G, & Feng M. (2022). Promoting antibody-dependent cellular phagocytosis for effective macrophage-based cancer immunotherapy. Science Advances, 8(11), eabl9171.

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Comprehensive Immune Cell Profiling

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Anti-human CD47 (clone B6H12, BD Biosciences), anti-mouse F4/80 (clone BM8, BioLegend), anti-Sirpα (clone P84, BioLegend), anti-mouse/human CD11b (clone M1/70, BioLegend), anti-mouse CD45 (clone 30-F11, BioLegend), anti-mouse MHC II (clone M5/114.15.2, BioLegend), anti-mouse CD206 (clone C068C2, BioLegend), anti-mouse CD80 (clone 16-10A1, BioLegend), anti-mouse CD86 (clone GL-1, BioLegend), anti-mouse PD-L1 (clone 10F.9G2, BioLegend), anti-mouse Gr1(clone RB6-8C5,BioLegend), anti-human CD14 (clone HCD14, BioLegend), and anti-human CD71 (clone CY1G4, BioLegend; clone OKT9, ThermoFisher; clone L01.1 and clone M-A712, BD Biosciences) were used for FACS analyses. Antibodies were Phycoerythrin (PE)-, PE/Cyanine7, APC, APC/Cyanine7, PerCP/Cyanine5.5, PE/Dazzle™ 594, Alexa Flour^® 700 or BV605 conjugated, or fluorophore-conjugated secondary antibodies were used. Annexin V (BD Biosciences), Sytox blue (ThermoFisher), 7-Aminoactinomycin D (7-AAD, ThermoFisher), or Zombie Violet™ Fixable Viability Kit (Biolegend) was used to exclude dead cells. Flow cytometry was performed using the BD LSRFortessa cell analyzers (BD).

Chen J., Cao X., Li B., Zhao Z., Chen S., Lai S.W., Muend S.A., Nossa G.K., Wang L., Guo W., Ye J., Lee P.P, & Feng M. (2021). Warburg Effect Is a Cancer Immune Evasion Mechanism Against Macrophage Immunosurveillance. Frontiers in Immunology, 11, 621757.

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