Horseradish peroxidase conjugated goat anti mouse secondary antibody
Horseradish peroxidase conjugated goat anti mouse secondary antibody is a laboratory reagent used for the detection and quantification of mouse primary antibodies in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry. It consists of a goat-derived secondary antibody that is chemically conjugated to the enzyme horseradish peroxidase, which catalyzes a colorimetric or chemiluminescent reaction for signal amplification and visualization.
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14 protocols using horseradish peroxidase conjugated goat anti mouse secondary antibody
Western Blot Analysis of PCNA in Nic-Cl Treated Cells
Immunoblotting for Activation-Induced Cytidine Deaminase
Western Blot Analysis of C. albicans Proteins
NPAS3 Protein Expression Analysis
Purification of Toxic Bacterial Proteins
Escherichia coli BL21 (DE3) strains carrying pET28b-based plasmids were grown in LB supplemented with kanamycin and induced with 0.2 mM IPTG for 12 h at 25°C. Specifically, in order to purify toxin HepT and HepT/MntA variants that are toxic, stationary cells (OD600 ∼ 1.0) were induced with 0.5 mM IPTG for only 2 h at 37°C (longer induction periods and IPTG addition at inoculation resulted in too much toxicity). Cells expressing the protein of interest were collected, and the His-tagged proteins were purified following the method described in a previous study (21 (link)). For the western blot assay, protein samples were transferred to a PVDF membrane (Millipore, Bedford, MA, USA), and performed with primary antibodies raised against a His-tag (Cell Signaling Technology, Danvers, MA, USA) and horseradish peroxidase-conjugated goat anti-mouse secondary antibodies (Bio-Rad, Richmond, CA, USA).
Lung Cancer Cell Line Cultivation
RalR Protein Expression Analysis
Comprehensive Western Blotting Protocol
Antibody Panel for Western Blot Analysis
Antibody-Based Protein Detection in Yeast
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