Agilent 6210 time of flight

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 6210 Time-of-Flight is a high-performance mass spectrometry instrument designed for accurate mass measurements. It employs time-of-flight technology to determine the mass-to-charge ratio of ionized molecules, enabling precise identification and characterization of a wide range of compounds.

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Lab products found in correlation

Eclipse plus c8 column, by Agilent Technologies (1 mentions) Agilent 1100 hplc, by Agilent Technologies (1 mentions) 6520 quadrupole time of flight, by Agilent Technologies (1 mentions) Hypersil rp c18 column, by Thermo Fisher Scientific (1 mentions) Dpx 400 nmr spectrometer, by Bruker (1 mentions) Sephadex lh 20, by GE Healthcare (1 mentions) Silica gel, by Qingdao Marine Chemical (1 mentions) Drx 600 spectrometer, by Bruker (1 mentions) Hplc system, by Hitachi (1 mentions) Gf254, by Qingdao Marine Chemical (1 mentions)

Close spelling variants of this product

Agilent 6210 Time-of-Flight LC/MS system Agilent 6210 time-of-flight mass spectrometer

4 protocols using agilent 6210 time of flight

Lipid and Aqueous Fraction MS Analysis of BALF and Plasma

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The lipid fraction positive mode MS conditions for the BALF and plasma samples were as follows: Agilent 6210 Time-of-Flight (TOF-MS) with dual ESI source, scan rate 2.03 spectra/second, mass range 60–1600 m/z, gas temperature 300 °C, gas flow 12.0 L/min, nebulizer 30 psi, skimmer 60 V, capillary voltage 4000 V, fragmentor 120 V, reference masses 121.050873 and 922.009798 (Agilent reference mix). The negative mode conditions were as follows: Agilent 6210 Time-of-Flight (TOF-MS) with dual ESI source, scan rate 2.02 spectra/second, mass range 60–1600 m/z, gas temperature 300 °C, gas flow 12.0 L/min, nebulizer 30 psi, skimmer 60 V, capillary voltage 4000 V, fragmentor 140 V, reference masses 112.985628 and 966.000725 (Agilent reference mix).
The aqueous fraction MS conditions for the BALF and plasma samples were as follows: Agilent 6520 Quadrupole Time-of-Flight (Q-TOF-MS) in positive ionization mode with ESI source, mass range 50–1700 m/z, scan rate 2.22 spectra/second, gas temperature 300 °C, gas flow 10.0 L/min, nebulizer 30 psi, skimmer 60 V, capillary voltage 4000 V, fragmentor 120 V, reference masses 121.050873 and 922.009798 (Agilent reference mix).

Cruickshank-Quinn C., Powell R., Jacobson S., Kechris K., Bowler R.P., Petrache I, & Reisdorph N. (2017). Metabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice. Scientific Reports, 7, 5108.

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HRMS Analysis of Environmental Samples

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Instrument methods for HRMS analysis are described in detail in Rager et al. (2016) (link) and Newton et al. (2018) (link). Briefly, samples were analyzed using an Agilent 1100 HPLC (Agilent Technologies, Palo Alto, CA) interfaced with an Agilent 6210 Time-of-Flight (TOF) mass spectrometer. Chromatographic separation was accomplished using an Eclipse Plus C8 column (2.1 × 50 mm, 3.5 μm; Agilent Technologies, Palo Alto, CA). The method consisted of the following conditions: 0.2 mL/min flow rate; column at 30 °C; mobile phases: A, ammonium formate buffer (0.4 mM) and DI water/methanol (95:5 v/v), and B, ammonium formate (0.4 mM) and methanol/DI water (95:5 v/v); gradient 0–25 min linear gradient from 75:25 A:B to 15:85 A:B; 25–40 min linear gradient from 15:85 A:B to 100% B; 40–50 hold at 100% B. The total run time was 45 min per sample, and ions from 100 to 1700 m/z were monitored in both positive and negative electrospray ionization (ESI) via separate injections. A reference compound mixture was constantly infused into the ion source for mass correction consisting of purine and hexakis (1H, 1H, 3H-tetrafluoropropoxy) phosphazene. Raw instrument data files were exported for further analysis and compound identification.

McEachran A.D., Hedgespeth M.L., Newton S.R., McMahen R., Strynar M., Shea D, & Nichols E.G. (2018). Comparison of emerging contaminants in receiving waters downstream of a conventional wastewater treatment plant and a forest-water reuse system. Environmental science and pollution research international, 25(13), 12451-12463.

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Spectroscopic Characterization of Compounds

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The HR-ESI-MS data were obtained on an Agilent 6210 time of flight LC-MS instrument (Agilent Technologies Inc., Palo Alto, CA, USA). NMR experiments were conducted on a Bruker DPX-400 NMR spectrometer (400 MHz for ¹H NMR and 100 MHz for ¹³C NMR) or Bruker DRX-600 spectrometer (600 MHz for ¹H NMR and 150 MHz for ¹³C NMR) (Bruker Corporation, Karlsruhe, Germany). The chemical shifts were given in δ (ppm) and referenced to the solvent signal (DMSO-d₆, δ_H 2.50, δ_C 39.5; acetone-d₆, δ_H 2.05, δ_C 29.8). Column chromatography (CC) was accomplished on silica gel (200–300 mesh, Qingdao Marine Chemical Inc., Qingdao, China), ODS (40–70 µm, Merck Company, Darmstadt, Germany) and Sephadex LH-20 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). Semi-preparative reverse-phase (RP) HPLC was performed on a Hitachi HPLC system with a L-7110 pump, a L-7420 UV/vis detector and an Hypersil RP-C₁₈ column (5 µm, 250 × 10.0 mm, Thermo Fisher Scientific, Waltham, MA, USA). Thin-layer chromatography (TLC) was conducted on silica gel GF254 (10–20 µm, Qingdao Marine Chemical Inc., Qingdao, China). All chemicals used were of HPLC grade or analytical grade.

Guo Z.K., Zhou Y.Q., Han H., Wang W., Xiang L., Deng X.Z., Ge H.M, & Jiao R.H. (2018). New Antibacterial Phenone Derivatives Asperphenone A–C from Mangrove-Derived Fungus Aspergillus sp. YHZ-1. Marine Drugs, 16(2), 45.

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Synthesis and Characterization of Paenipeptin C'

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Paenipeptin C′ was synthesized by solid-phase peptide synthesis (SPPS) and purified by HPLC to homogeneity (> 95% purity) through custom peptide service (Genscript Inc., Piscataway, NJ). The accurate mass of paenipeptin C′ was determined using liquid chromatography mass spectrometer (Agilent 6210 Time-of-Flight, Agilent Technologies, Santa Clara, CA). The theoretical mass of paenipeptin C′ [M + H]⁺ ion is 1116.7510 m/z, which is consistent with the measured mass of 1116.7518 m/z.

Moon S.H, & Huang E. (2019). Novel linear lipopeptide paenipeptin C′ binds to lipopolysaccharides and lipoteichoic acid and exerts bactericidal activity by the disruption of cytoplasmic membrane. BMC Microbiology, 19, 6.

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