Pancreas and livers were collected and cut into small pieces, fixed in 10% natural buffered formaldehyde (formalin). Following fixation, specimens were dehydrated, embedded in paraffin, and then sectioned to 5 µm thicknesses using a semi-automated rotatory microtome. Sections were stained with hematoxylin and eosin. Finally, histopathological changes were examined under phase contrast microscope (Olympus B x 41, Japan) and images were captured at a magnification of 40× using a computer-assisted image analyzer (Nikon H600L, Nikon DS camera control Unit DS-U2, Version 4.4). The number of hepatocytes and Islets of Langerhans (IL), the diameter of the cores of hepatocytes and areas of IL were determined. For this reason, quantitative analyses were carried out using ImageJ software 1.50i (wayne Rasband National Institutes of Health, USA). Morphometric analysis was performed with ImageJ software 1.50i (wayne Rasband National Institutes of Health, USA) [14 (link)-16 (link)].
Ds camera control unit ds u2 version 4
Manufactured by Nikon
Sourced in Japan
The Nikon DS camera control Unit DS-U2, Version 4.4 is a device that enables the control and operation of Nikon digital cameras for use in laboratory and research applications. It provides an interface for connecting the camera to a computer system and allows for the remote control of camera functions and settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ds camera control unit ds u2 version 4
1
Histomorphometric Analysis of Liver and Pancreas
2
Histopathological Evaluation of Liver Tissue
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Liver was excised immediately following sacrifice, washed with a phosphate buffer solution, and then fixed in 10% formalin. Tissues were dehydrated through graded series of alcohol, cleared in xylene, and embedded in paraffin wax. Tissues were then cut into sections of 5 μm in thickness using a microtome (Histo-line laboratories, ARM-3600, Viabrembo, Milano, Italy) and stained with hematoxylin-eosin (H&E). Histopathological changes were examined under phase contrast microscope (Nikon H600L, Japan) and images were captured at magnification of 40× using a computer-assisted image analyzer (Nikon H600L, Nikon DS camera control Unit DS-U2, Version 4.4). Histopathological changes such as necrosis, sinusoidal hyperemia, and connective tissue inflammation of the portal region were scored by three independent observers following the description by Guven et al. [26 (link)] (0: normal, no changes, +: mild, ++: moderate, and +++: severe changes).
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