Tnf alpha elisa kit

Manufactured by Thermo Fisher Scientific

Sourced in Germany

The TNF alpha ELISA kit is a laboratory assay used to quantify the levels of tumor necrosis factor alpha (TNF-alpha) in biological samples. TNF-alpha is a pro-inflammatory cytokine that plays a role in various physiological and pathological processes. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of TNF-alpha in the sample.

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2 protocols using tnf alpha elisa kit

Gastric Ulcer Protective Assays

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All the reagents below were of analytical grade, and the stock solutions and buffers were prepared with Milli-Q water (Millipore, Billerica, MA, USA). Ethanol, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), TPTZ (2,4,6-tris (2-pyridyl)-s-triazine), tris(hydroxymethyl)aminomethane, sodium chloride, ascorbic acid, Griess reagent, lansoprazole, ranitidine hydrochloride, 2-thiobarbituric acid, EDTA (ethylene diamine tetraacetic acid), N-acetylcysteine, quercetin, gallic acid, ellagic acid, tannic acid and catechin, IU urease (jack bean urease, type III), Alcian blue, N-ethylmaleimide, N_ω-nitro-L-arginine methyl ester, ranitidine, carbenoxolone were obtained from Sigma-Aldrich (St. Louis, MO, USA). Trolox is obtained from Merck, Düsseldorf, Germany; TNF alpha ELISA kit is from eBioscience, San Diego, CA, USA. Historesin is obtained from Leica Biosystems, Wetzlar, Germany, and acetic acid and glucose were obtained from Vetec, Duque de Caxias, Rio de Janeiro, Brazil. Ethyl ether, formaldehyde, and phenolphthalein are from FMaia, Cotia, São Paulo, Brazil; xylazine and ketamine are obtained from Vetbrands, Paulínia, São Paulo, Brazil. Ki-67 protein (code: sc-23900) and BrdU protein (code: sc-32323) are from Santa Cruz Biotechnology, Santa Cruz, CA, USA.

Brito S.A., de Almeida C.L., de Santana T.I., da Silva Oliveira A.R., do Nascimento Figueiredo J.C., Souza I.T., de Almeida L.L., da Silva M.V., Borges A.S., de Medeiros J.W., da Costa Silva Neto J., de Cássia Ribeiro Gonçalves R., Kitagawa R.R., Sant'Ana A.E., Rolim L.A., de Menezes I.R., da Silva T.G., Caldas G.F, & Wanderley A.G. (2018). Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L.. Oxidative Medicine and Cellular Longevity, 2018, 1731459.

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Evaluating Anti-Inflammatory Agents

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N-acetyl cysteine (NAC), Lipopolysaccharides from Escherichia coli (LPS), 2-mercaptoethenol, DL-dithiothreitol (DTT), tris(2-carboxyethyl)phosphine (TCEP), 2, 2-Azobisisobutyronitrile (AIBN), Poly(ethylene glycol)methacrylate (Mn = 360 Da) and Thiazolyl Blue Tetrazolium Bromide (MTT) were purchased from Sigma–Aldrich. Aldrithiol-2 and Silica gel (Spherical, 100 µm) were purchased from Tokyo Chemical Industry Co. Interleukin-1 beta ELISA kit, Iba-1 antibody and Alexa Fluor 568 to Rabbit IgG secondary Antibody were purchased from abcam, Nitrate/nitrite colorimetric assay kit was purchased from Cayman chemical and TNF alpha ELISA kit was purchased from eBioscience. Cell tracker dyes were purchased from Thermo Fisher Scientific. Penicillin (10,000 U/mL), streptomycin (10,000 µg/mL), 0.25% trypsin-EDTA, Dulbecco's Modified Eagle Medium (with L-glutamine), DMEM/F12 (1:1) (with L-glutamine), MEM-Non–essential amino acids 100X, sodium pyruvate 100 mM and fetal bovine serum (FBS) were obtained from Gibco.

Markoutsa E, & Xu P. (2017). Redox potential sensitive N-acetyl cysteine-prodrug nanoparticles inhibit the activation of microglia and improve neuronal survival. Molecular pharmaceutics, 14(5), 1591-1600.

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