The largest database of trusted experimental protocols

2 protocols using α tocopherol succinate

1

Formulation and Characterization of Doxorubicin-Loaded Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Doxorubicin hydrochloride (DOX) was purchased from ACIC Chemicals (Brantford, ON, Canada). α-Tocopherol succinate (TS), α–Tocopherol polyethylene glycol 1000 succinate (TPGS), and Bovine Serum Albumin (BSA) were purchased from Sigma-Aldrich (São Paulo, Brazil). Poly(L-lactide-co-glycolide) 50:50 (PLGA) was obtained from Evonik Health Care (Essen, Germany). Soybean Lecithin was purchased from Lipoid GmbH (Ludwigshafen, Germany). Triethanolamine (TEA) was obtained from Merck (Darmstadt, Germany). RPMI1640 medium, Dulbecco’s Modified Eagle’s Medium (DMEM), Minimum Essential Medium (MEM), streptomycin, penicillin, and amphotericin B (PSA), fetal bovine serum (FBS), and trypsin EDTA (0.25%) were purchased from Gibco-Invitrogen (Grand Island, NE, USA). All other chemicals were analytical grade.
+ Open protocol
+ Expand
2

Curcumin and DNA Damage Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Curcumin (98% pure), BaP, α-tocopherol succinate, and dimethylsulfoxide (DMSO) were purchased from Sigma Chemical Co. (MO, USA). Curcumin and α-tocopherol were dissolved in DMSO at a concentration of 50 μM and all the solutions were stored in a dark-colored bottle at −80°C as a stock solution. The stock was diluted to the required concentration with growth media immediately before use. 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) was purchased from Usb Corporation (CA, USA). DNeasy Blood and Tissue kits were purchased from QIAGEN (MD, USA). Acetonitrile (ACN) and ammonium formate (AF) were purchased from Merck (Darmstadt, Germany) and HCl (1 N) was from Fisher Scientific (Fair Lawn, NJ). BaP-tetrol standards, including r-7,c-10,t-8,t-9-tetrahydroxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BaP tetrol I-1), and r-7,t-9,t-10,t-8-tetrahydroxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BaP tetrol I-2) were purchased from the MRIGlobal Chemical Carcinogen Reference Standard Repository (MO, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!