Mouse anti horse cd44 fluorescein isothiocyanate fitc clone cvs18

The cell samples between the third (P3) and the fourth passage (P4) were washed with a PBS and distributed at 2 × 10⁵ cells by tube. The cells were centrifuged (300 r/min, 5 min) and the pellets were resuspended in PBS containing of the antibodies [mouse anti-rat CD90-phycoerythrin - PE (clone OX-7; BD, San Jose, CA, United States)/mouse anti-horse CD44-fluorescein isothiocyanate - FITC (clone CVS18; AbD Serotec, Oxford, United Kingdom)] and mouse anti-human CD34-FITC (clone 581; BD)]. Anti- IgG1-PE and anti-IgG1-FITC were used as control isotypes to calibrate the cytometer. After 45 min incubation (4 °C, in the dark), the cells were washed with PBS and centrifugated (300 r/min, 5 min). Pellets were resuspended in 300 μL of PBS and tubes analyzed to flow cytometer (FACSCalibur^® - Becton Dickinson, San Jose, CA, United States) and Cell-Quest software (Becton Dickin- son, San Jose, CA, United States). The protocols were performed according to the manufacturer’s instructions.