Sc30 optical microscope accessory cmos color camera

Formalin-fixed samples were processed through paraffin embedment, sectioned at 5 µm (interscapular BAT, visceral (PGAT) and subcutaneous (SQAT) WAT) and stained with H&E. Sections were evaluated via an Olympus BX34 photomicroscope (Olympus, Melville, NY, USA) and images were taken via an Olympus SC30 Optical Microscope Accessory CMOS color camera. Adipocyte size was calculated from three independent regions of the same 40× objective fields for SQAT, PGAT, and interscapular BAT depots (50 adipocytes/animal). Cross-sectional areas of the adipocytes were obtained from perimeter tracings using Image J software, as previously described [51 (link)]. An investigator blinded to the groups performed all procedures.

Formalin-fixed samples were processed through paraffin embedment, sectioned at 5 µm (visceral (PGAT), subcutaneous (SQAT) WAT, and interscapular BAT) and stained with H&E. Sections were evaluated via an Olympus BX34 photomicroscope (Olympus, Melville, NY, United States) and images were taken via an Olympus SC30 Optical Microscope Accessory CMOS color camera. Adipocyte size was calculated from three independent regions of the same ×40 objective fields for SQAT, PGAT, and interscapular BAT depots (∼75 adipocytes/animal). Cross-sectional areas of the adipocytes were obtained from perimeter tracings using ImageJ software, as previously described (Wainright et al., 2015 (link)). An investigator blinded to the groups performed all procedures. Note that the representative images from the WT control mice were previously published in the investigation of sex differences in CL-mediated browning (Queathem).

Formalin-fixed samples were processed through paraffin embedment, sectioned at 5 μm (interscapular BAT, visceral (PGAT) and subcutaneous (SQAT) WAT) and stained in a 1:1200 dilution with UCP1 antibody (#U6382, 1:1000, Sigma-Aldrich; secondary antibody, #K400311-2, Envision Rabbit, Agilent) for 30 min with a heat-induced epitope retrieval (HIER) pretreatment, using DAKO brand citrate in a decloaking chamber. Sections were evaluated via an Olympus BX34 photomicroscope (Olympus, Melville, NY) and images were taken via an Olympus SC30 Optical Microscope Accessory CMOS color camera. Adipocyte size was calculated from three independent regions of the same 40× objective fields for SQAT, PGAT, and interscapular BAT depots (50 adipocytes/animal). Cross-sectional areas of the adipocytes were obtained from perimeter tracings using Image J software as previously described (Wainright et al., 2015 (link)). An investigator blinded to the groups performed all procedures.