The control (WT) C. albicans QMY23 (his1Δ/his1Δ, leu2Δ:C. dubliniensis HIS1/leu2Δ:C. maltose LEU2, URA3/ura3Δ:imm434, IRO1/iro1Δ:imm434) and the phosphatase gene deletion mutant (KO) (ura3Δ-iro1Δ:imm434/URA3-IRO1, his1Δ/his1Δ, leu2Δ/leu2Δ, ppz1Δ:C. dubliniensis HIS1/ppz1Δ:C. maltosa LEU2) strains were constructed earlier by Mitrovich et al. [18 (link)] and Ádám et al. [14 (link)], respectively. These strains were maintained and cultured on yeast extract-peptone-dextrose (YPD) agar (1% yeast extract (Alfa Aesar, Kandel, Germany), 2% mycological peptone (HiMedia, Mumbai, India), 2% dextrose (VWR International LLC., Debrecen, Hungary) and ±2% agar (VWR International LLC., Hungary), pH 5.6) as described earlier [17 (link)]. Unless otherwise indicated, all chemicals were purchased from Sigma-Aldrich Hungary Ltd., Budapest, Hungary.
Mycological peptone
Manufactured by HiMedia
Sourced in India
Mycological peptone is a high-quality nutrient source used in microbiological media. It provides essential amino acids, peptides, and other growth factors to support the cultivation of fungi and other microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
Yeast extract, by HiMedia (3 mentions)
Malt extract, by HiMedia (3 mentions)
Lithium bromide, by Merck Group (1 mentions)
Nahco3, by Merck Group (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (1 mentions)
Veratryl alcohol, by Merck Group (1 mentions)
2 6 dichlorophenolindophenol, by Merck Group (1 mentions)
2 6 dimethoxyphenol, by Merck Group (1 mentions)
D glucose, by Merck Group (1 mentions)
Pcr master mix, by Promega (1 mentions)
Dichloran rose bengal chloramphenicol agar, by Thermo Fisher Scientific (1 mentions)
Czapek dox agar, by Thermo Fisher Scientific (1 mentions)
4 protocols using mycological peptone
1
Construction and Maintenance of Candida Strains
2
Sophorolipid Biosynthesis from Candida bombicola
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Silk
fibers of Bombyx mori silkworm used
in this work were procured
from Central Sericultural Research and Training Institute, Mysuru,
India. Chemicals required in silk processing, i.e., NaHCO3 and lithium bromide, were procured from Merck and Sigma Aldrich,
respectively. Different media components used for yeast culture and
fermentation, namely, malt extract, glucose, yeast extract, and mycological
peptone as well as nutrient agar powder were obtained from Himedia,
India. Minor media components (salts) MgSO4, Na2HPO4, NaH2PO4, and (NH4)2SO4 were purchased from SRL, India. Lauric
acid (LA), used as a precursor for LASL production, was procured from
Loba Chemicals.
The sophorolipid product (LASL) was synthesized
using a non-pathogenic yeast Candida bombicola (ATCC 22214) maintained on MGYP medium slants (malt extract, 0.3
g %; glucose, 10 g %; yeast extract, 0.3 g %; mycological peptone,
0.5 g %) for growth at 28 °C and stored at 4 °C.
fibers of Bombyx mori silkworm used
in this work were procured
from Central Sericultural Research and Training Institute, Mysuru,
India. Chemicals required in silk processing, i.e., NaHCO3 and lithium bromide, were procured from Merck and Sigma Aldrich,
respectively. Different media components used for yeast culture and
fermentation, namely, malt extract, glucose, yeast extract, and mycological
peptone as well as nutrient agar powder were obtained from Himedia,
India. Minor media components (salts) MgSO4, Na2HPO4, NaH2PO4, and (NH4)2SO4 were purchased from SRL, India. Lauric
acid (LA), used as a precursor for LASL production, was procured from
Loba Chemicals.
The sophorolipid product (LASL) was synthesized
using a non-pathogenic yeast Candida bombicola (ATCC 22214) maintained on MGYP medium slants (malt extract, 0.3
g %; glucose, 10 g %; yeast extract, 0.3 g %; mycological peptone,
0.5 g %) for growth at 28 °C and stored at 4 °C.
3
Enzymatic Decolorization of Textile Dyes
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All chemicals were of analytical grade. Cellulose (microcrystalline) powder, malt extract, agar, yeast extract, and mycological peptone were purchased from Hi-Media Laboratories Pvt. Ltd. (Mumbai, India). All substrates (ABTS, 2,6-Dichlorophenol-indophenol or DCIP, 2,6-Dimethoxyphenol, Veratryl alcohol) used for measurement of enzyme activities were procured from Sigma-Aldrich Corp. (St. Louis, USA). Reactive blue (RB) 21, Reactive orange (RO) 16, Reactive red (RR) 198, Reactive violet (RV) 5 and Reactive yellow (RY) 42 were obtained from Department of Textile Technology, Indian Institute of Technology Delhi, New Delhi India. The solvents were from J.T. Baker (U.S.A.).
4
Fungal Cultivation and DNA Extraction
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Mycological peptone, microbiological grade agar, malt extract and yeast extract were purchased from Himedia Laboratories (Mumbai, India); Dichloran Rose Bengal Chloramphenicol Agar (DRBC) and Czapek Dox Agar (CZ) ready prepared culture media were purchased from Oxoid (Basingstoke, U.K.); D(+) glucose was purchased from Sigma-Aldrich (Merck, Darmstadt, Germany); salts (
were purchased form Carlo Erba Reagents S. r. l. (Milano, Italy); a DNA "Plant II" extraction kit was purchased from Macherey-Nagel (Düren, Germany); PCR master mix was purchased from Promega Corporation (Madison, Wisconsin, USA); an Exosap cleaning kit was purchased from Euroclone (Pero (MI), Italy).
were purchased form Carlo Erba Reagents S. r. l. (Milano, Italy); a DNA "Plant II" extraction kit was purchased from Macherey-Nagel (Düren, Germany); PCR master mix was purchased from Promega Corporation (Madison, Wisconsin, USA); an Exosap cleaning kit was purchased from Euroclone (Pero (MI), Italy).
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