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Pi staining kit

Manufactured by Abcam
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Sourced in United Kingdom

The PI staining kit is a laboratory tool used to detect and quantify DNA content in cells. It contains the fluorescent dye propidium iodide (PI), which binds to DNA, allowing for the analysis of cellular DNA levels.

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Lab products found in correlation

Fetal bovine serum (fbs), by PAN Biotech (1 mentions) Annexin 5, by Abcam (1 mentions) Dulbecco s modified eagle s medium, by PAN Biotech (1 mentions) Alexa fluor 488 dye, by Thermo Fisher Scientific (1 mentions) Pi rnase a, by Abcam (1 mentions) Cytexpert software, by Beckman Coulter (1 mentions)

Spelling variants of this product

Annexin-V FITC/PI staining kit (2 citations) Annexin V/PI double staining kit (2 citations) Annexin V-APC/PI Dual Staining Apoptosis Assay Kit (2 citations)

2 protocols using pi staining kit

1

Fluorescence-Based Cellular Assays

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Dulbecco’s modified Eagle’s medium (DMEM) and Fetal bovine serum (FBS) was purchased from PAN-Biotech GmbH (Am Gewerbepark, Aidenbach). Green-fluorescent Alexa Fluor 488 dye purchased from Molecular probes, Invitrogen. 2′,7′-dichlorofluorescein diacetate (DCF-DA), Di-hydro Rhodamine (DHR) from Invitrogen (Bangalore). 4′,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI), Amplex red Diaminorhodamine-4M-AM (DAR-4M-AM), were purchased from Sigma Chemical Co (St. Louis, MO) USA. Annexin V and PI staining kit was purchased from Abcam. All other chemicals used in this study were of reagent grade and obtained commercially.
Balaguru U.M., Sundaresan L., Manivannan J., Majunathan R., Mani K., Swaminathan A., Venkatesan S., Kasiviswanathan D, & Chatterjee S. (2016). Disturbed flow mediated modulation of shear forces on endothelial plane: A proposed model for studying endothelium around atherosclerotic plaques. Scientific Reports, 6, 27304.
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2

Cell Cycle Analysis of PFN1 in DNA Damage

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The cell cycle stage depending on the status of PFN1 in response to DNA damage was analyzed by using propidium iodide (PI) staining kit (Abcam, UK). Cells were harvested and fixed in 70% ice-cold ethanol overnight, washed two times with PBS, and resuspended in PI/RNase A (Abcam, UK) buffer for 15 min at room temperature. Cells were then examined by flow cytometer (Beckman Coulter, USA) and the data were analyzed using CytExpert software (Beckman Coulter, USA).
Lee C.J., Yoon M.J., Kim D.H., Kim T.U, & Kang Y.J. (2021). Profilin-1; a novel regulator of DNA damage response and repair machinery in keratinocytes. Molecular Biology Reports, 48(2), 1439-1452.
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