This protein was determined in kidney tissue by KIM-1/TIM-1 Rat ELISA Kit (no. ab119597), according to the manufacturer’s instructions (Abcam, USA).
Kim 1 tim 1 rat elisa kit
Manufactured by Abcam
Sourced in United States, United Kingdom
The KIM-1/TIM-1 Rat ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of rat kidney injury molecule-1 (KIM-1) and T-cell immunoglobulin and mucin domain-containing protein 1 (TIM-1) levels in biological samples. The kit utilizes a specific antibody coated on a microplate to capture the target analyte from the samples, which is then detected using a biotinylated detection antibody and an enzyme-conjugated streptavidin. The assay provides a reliable and sensitive method for quantifying KIM-1 and TIM-1 in rat samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using kim 1 tim 1 rat elisa kit
1
Kidney Protein Quantification using ELISA
2
Biochemical and Cytokine Profiling in Rat Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentrations of creatinine and urea in serum were determined using a modified Jaffe colorimetric method and the urease enzymatic method, respectively, by means of commercial kits and spectrophotometry with an automatic analyser ILAB-Aries (ILab-300 Plus; Instrumentation Laboratory, Bedford, MA, USA).
The serum levels of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α were determined using a sandwich enzyme-linked immunoassay protocol development kit (Peprotech, Mexico City, Mexico). The optical density was measured at 405/620 nm using a microplate-reading spectrophotometer (Mutilskan FC, Thermo Fischer Scientific Oy, Vantaa, Finland). The results are expressed as ng/mL.
For quantitative detection of rat serum NGAL and KIM-1, Abcam one-step ELISA kits were used (Lipocalin-2/NGAL Rat ELISA Kit, Abcam 119602, Abcam, Cambridge, UK; KIM-1/TIM-1 Rat ELISA Kit Abcam 119597, Cambridge, MA, USA, respectively). Cys-C was measured using ELISA kits from R&D Systems (Quantikine ELISA; Mouse/Rat Cystatin C Immunoassay, R&D Systems, Minneapolis, MN, USA).
The serum levels of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α were determined using a sandwich enzyme-linked immunoassay protocol development kit (Peprotech, Mexico City, Mexico). The optical density was measured at 405/620 nm using a microplate-reading spectrophotometer (Mutilskan FC, Thermo Fischer Scientific Oy, Vantaa, Finland). The results are expressed as ng/mL.
For quantitative detection of rat serum NGAL and KIM-1, Abcam one-step ELISA kits were used (Lipocalin-2/NGAL Rat ELISA Kit, Abcam 119602, Abcam, Cambridge, UK; KIM-1/TIM-1 Rat ELISA Kit Abcam 119597, Cambridge, MA, USA, respectively). Cys-C was measured using ELISA kits from R&D Systems (Quantikine ELISA; Mouse/Rat Cystatin C Immunoassay, R&D Systems, Minneapolis, MN, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!