Trametinib and U0126 were purchased from MedChemExpress (Monmouth Junction, NJ, United States). Dimethyl sulfoxide was used to dissolve Trametinib powder to an initial concentration of 10 mM and preserved in −80°C. The CCK-8 kit was obtained from Dojindo (Rockville, MD, United States). The Annexin V-FITC/PI apoptosis detection kit and Annexin V-PE/7-ADD apoptosis detection kit were purchased from Nanjing Vazyme Biotech (Nanjing, China). Penicillin and streptomycin were obtained from Gibco (Thermo Fisher Scientific, Waltham, MA, United States). DMEM were purchased from CORNING (Corning Life Sciences, Bedford, MA, Unied States). The fetal bovine serum (FBS) was purchased from TBD (Tianjin, China). Lipofectamine 3,000 and TRIzol™ Reagent were obtained from Life Technologies (Carlsbad, CA, United States). XF96 seahorse assay plate was purchased from Agilent (Santa Clara, CA, United States). The antibodies used were as follows: anti-PKM2 antibody (#15822-AP), anti-c-myc antibody (#67447-1-lg), anti-GLUT1 antibody (#21829-1-AP), anti-LDHA antibody (#19987-1-AP), anti-β-actin antibody (#66009-1-lg), MEK1/2 polyclonal antibody (#11049-1-AP), ERK1/2 polyclonal antibody (#16443-1-AP), and anti-PCNA antibody (10205-2-AP) from Proteintech (Wuhan, China); and anti-Ki67 antibody (ab279653) from Abcam (Cambridge, United Kingdom).
Anti ldha antibody
Manufactured by Proteintech
Sourced in China, United States
The Anti-LDHA antibody is a laboratory tool designed to detect the presence and measure the levels of the LDHA (Lactate Dehydrogenase A) protein in biological samples. LDHA is an enzyme involved in cellular metabolism and is a common target of interest in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Trametinib, by MedChemExpress (1 mentions)
Anti c myc antibody, by Proteintech (1 mentions)
Anti pkm2 antibody, by Proteintech (1 mentions)
Anti glut1 antibody, by Proteintech (1 mentions)
Annexin 5 pe 7 add apoptosis detection kit, by Vazyme (1 mentions)
Anti β actin antibody, by Proteintech (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Cck 8 kit, by Dojindo Laboratories (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Vazyme (1 mentions)
U0126, by MedChemExpress (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Goat anti rabbit igg, by Agilent Technologies (1 mentions)
Ifn γ, by Fujifilm (1 mentions)
Anti hif 1α antibody, by Cayman Chemical (1 mentions)
Taqman gene expression master mix, by Thermo Fisher Scientific (1 mentions)
Revertra ace qpcr rt kit, by Toyobo (1 mentions)
2 protocols using anti ldha antibody
1
Trametinib and U0126 Proliferation Assay
2
Hypoxia-Inducible Factor Protein Analysis
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EnVision™ kits, horseradish-peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) and goat anti-rabbit IgG were obtained from Dako/Agilent (Santa Clara, CA, USA). A polyclonal anti-HIF-1α antibody was from Cayman (Ann Arbor, MI, USA). Anti-HIF-2 and anti-HIF-1β antibodies were obtained from Novus Biologicals (Litteton, CO, USA), and anti-LDH-A antibody was from Proteintech (Rosemont, IL, USA). Dulbecco’s modified Eagle’s medium (DMEM) with pyruvate and IFN-γ were obtained from Wako Pure Chemical Industries Ltd (Osaka, Japan) and DMEM without pyruvate was obtained from Nacalai Tesque (Kyoto, Japan). Fetal bovine serum (FBS) was from Equitech Bio (Kerrville, TX, USA). An anti-β-actin antibody and oxamate were purchased from Sigma (St Louis, MO, USA). Isogen was obtained from Nippon Gene (Toyama, Japan) and the ReverTra Ace qPCR RT Kit was from Toyobo (Osaka, Japan). The Ambion PARIS™ Kit and TaqMan gene expression master mix were purchased from Applied Biosystems (Carlsbad, CA, USA). SYBR Green real-time PCR master mix was purchased from Roche/Nippon Genetics (Tokyo, Japan). [14C]-Pyruvate was obtained from the Japan Radioisotope Association (Tokyo, Japan).
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