A Shimadzu UHPLC system consisting of a CBM-20A system controller, LC-20AD XR pumps, DGU-20A3 degasser, SIL-20AC XR auto sampler, and CTO-20A column oven coupled with an electrospray ionization (ESI) interface equipped with an LCMS-8030 triple quadrupole mass spectrometer (Shimadzu, Kyoto, Japan) were utilized for the separation and detection of bioactive compounds in Yin-Chen-Hao-Tang and carbamazepine (IS). The remote-controlled software for the Shimadzu UHPLC-MS/MS system (Kyoto, Japan) was LabSolutions v. 5.60 SP1. Statistical calculations were performed using Microsoft Excel.
Uhplc ms ms system
Manufactured by Shimadzu
Sourced in Japan
The UHPLC-MS/MS system is a high-performance liquid chromatography-tandem mass spectrometry instrument designed for advanced analytical applications. It combines ultra-high-performance liquid chromatography (UHPLC) technology with a sensitive and precise mass spectrometry (MS/MS) detection system. This system provides rapid and accurate separation, identification, and quantification of a wide range of analytes in complex samples.
Automatically generated - may contain errors
Lab products found in correlation
Lcms 8030 triple quadrupole mass spectrometer, by Shimadzu (1 mentions)
Lc 20adxr pump, by Shimadzu (1 mentions)
Cto 20a column oven, by Shimadzu (1 mentions)
Sil 20acxr autosampler, by Shimadzu (1 mentions)
Cbm 20a system controller, by Shimadzu (1 mentions)
Dgu 20a3 degasser, by Shimadzu (1 mentions)
Uhplc system, by Shimadzu (1 mentions)
Lcms 8045, by Shimadzu (1 mentions)
3 protocols using uhplc ms ms system
1
Yin-Chen-Hao-Tang Bioactive Compound Analysis
2
UHPLC-MS/MS Method for Remdesivir
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The UHPLC parameters utilized were the same as mentioned in UHPLC-PDA method parameters section 2.4.1 . The RDV was also simultaneously monitored using a Shimadzu UHPLC-MS/MS system model LCMS-8045 (Tokyo, Japan) equipped with triple quadrupole with an electrospray ionization (ESI) source. The ESI positive ion mode was used to detect RDV with the scheduled multiple reaction monitoring (MRM) mode by the protonated RDV molecule used as precursor ion. The ESI ion source heating block temperature was set at 300 °C, DL temperature was set at 250 °C, the interface temperature was set at 300 °C, the heating gas flow rate at 10 L/min, the nebulizing gas flow rate at 3 L/ min, and drying gas flow 10 L/min for the RDV analysis. The ion transitions of RDV were m/z 603.1→m/z 402.20 and m/z 199.90, dwell time 100 msec for both ions, collision energy (CE) are -17 and -42, Q1 pre-bias (V) -22.0 and Q3 pre-bias (V) -15.0 and -20.0 were used to monitor.
3
Hair Steroid Hormone Analysis
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Hair samples were collected scalp-near from a posterior vertex position at the back of the head. As the average hair growth rate is 1 cm/month [32] (link), a collected one centimeter hair segment would contain steroid hormones deposited over the previous month prior to hair sampling. After hair collection, the samples were stored at room temperature in aluminum foil [33] (link) and analyzed in laboratories of the Institute of Biomedical Sciences, Faculty of Medicine, Vilnius University. Hair hormone (cortisol, cortisone, DHEA) concentrations were detected using the ultra-high-performance liquid mass spectrometry (UHPLC-MS/MS) system (Shimadzu Corporation, Kyoto, Japan). Full details of the extraction procedure and analysis have been reported elsewhere by Mazeikiene et al. [34] (link).
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