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Anti chicken igy h l alexa fluor 488

Manufactured by Abcam

Anti-Chicken IgY H&L Alexa Fluor 488 is a secondary antibody conjugated with Alexa Fluor 488 dye. It is designed to detect and bind to chicken immunoglobulin Y (IgY) and its heavy and light chains.

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2 protocols using anti chicken igy h l alexa fluor 488

1

Cryosection Immunostaining in Zebrafish

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The cryosection immunostaining was performed as previously described54 (link). The primary antibodies were anti-GFAP (DAKO, Z0334), anti-GFP (Abcam, ab13970), anti-PCNA (Sigma, P8825), anti-PSA-NCAM (Chemico, MAB5324), and anti-H2A.XS139ph (Genetex, GTX127340). The zebrafish p53 polyclonal antibody was generated by HuaAn Biotechnology (Hangzhou, China) as previously described. The secondary antibodies were anti-Rabbit IgG H&L Dylight 549 (EarthOx, E032320), anti-Rabbit IgG H&L Alexa Fluor 647 (Abcam, ab150143), anti-Chicken IgY H&L Alexa Fluor 488 (Abcam, ab150169), anti-mouse IgG H&L Alexa Fluor 488 (Abcam, ab150113), and anti-mouse IgG H&L Alexa Fluor 647 (Abcam, ab150115). Nuclei were stained by DAPI (BYT, C1002).
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2

Cryosection Immunostaining Protocol for Tissue Analysis

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The cryosection was performed as previously described [35 (link)]. For immunostaining, the primary antibodies were anti-MF20 (DSHB, AB 2147781, 1:50), anti-Glut1 (ABclonal, A6982, 1:200), anti-cmyc (ABclonal, A1309, 1:200), anti-GFP (Abcam, ab13970). The second antibodiess were anti-mouse IgG H&L Alexa Fluor 488 (Abcam, ab150113), anti-Chicken IgY H&L Alexa Fluor 488 (Abcam, ab150169), Dylight 549-conjugated anti-rabbit IgG H&L (EarthOx, E032320), anti-Rabbit IgG H&L Alexa Fluor 647 (Abcam, ab150143), anti-mouse IgG H&L Alexa Fluor 647 (Abcam, ab150115). Nuclei were stained by DAPI (BYT, C1002).
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