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Ft ir prestige 21 spectrophotometer

Manufactured by Shimadzu
Sourced in Japan

The FT-IR Prestige-21 spectrophotometer is a Fourier Transform Infrared (FT-IR) spectrometer manufactured by Shimadzu. It is a laboratory instrument used for the analysis of chemical compounds by measuring the absorption or transmission of infrared radiation.

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5 protocols using ft ir prestige 21 spectrophotometer

1

Characterization of PMMA Nanoparticles and CaCO3 Crystals

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UV–vis spectra of
poly(methyl methacrylate) nanoparticles (PMMA-NPs) were recorded using
a UV-1800 Shimadzu spectrophotometer. The fluorescence spectra were
recorded on an Agilent Cary Eclipse Fluorescence spectrophotometer.
The images were captured under differential interference contrast
(DIC) and fluorescein isothiocyanate (FITC) filters. Hydrodynamic
radii and ζ-potential of the particles in water were measured
using a Malvern Zeta Sizer instrument. The morphology of CaCO3 crystals collected on the coverslips was established using
an Olympus EVOS7000 optical fluorescence microscope and a JEOL JSM-6701F
scanning electron microscope (SEM). The composition of CaCO3 was analyzed using a Shimadzu model Fourier transform infrared (FTIR)
Prestige-21 spectrophotometer within the range of 4000–400
cm–1. The crystal lattice structure of the CaCO3 samples was investigated using a Bruker D8 Advance Powder
Crystal X-ray diffractometer with Cu Kα radiation (λ =
0.154 nm) within a 2θ range of 10–70° and source
at 40 kV and 40 mA.
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2

Comprehensive Characterization of Bio Nanocarbon

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Characterisation studies were carried out to investigate the properties of the prepared bio nanocarbon. The morphological properties of the synthesised materials were obtained using transmission electron microscopy (TEM), which was investigated with an energy-filtered EFTEM Libra 120—Carl Zeiss instrument (Oberkochen, Germany). Dynamic light scattering (DLS) was conducted on a Malvern Zetasizer Nano-ZS Ver. 7.11 (Malvern Instruments, Malvern, UK) for the evaluation of the particle sizes. Furthermore, the zeta potential measurement was conducted using a Malvern Zetasizer Nano Ver 7.11 (Malvern Instruments, Malvern, UK). The elemental composition of the activated bionanocarbon was analysed with Energy Dispersive X-ray (EDX) using field emission scanning electron microscope FESEM/EDX (FEI Quanta FEG 650, Thermo Fisher Scientific, Eindhoven, The Netherlands). The functional group analysis of bionanocarbon and nonwoven kenaf fibre was obtained using an FT-IR Prestige-21 spectrophotometer (Shimadzu, Chiyoda-ku, Tokyo, Japan). The morphologies of the nonwoven kenaf fibre were analysed using FESEM (EVO MA 10, Carl-ZEISS SMT, Oberkochen, Germany).
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3

Characterization of Biogenic Silver Nanoparticles

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A UV-vis spectrophotometer (Perkin Elmer, LS 55) was used to characterize the synthesized AME-AgNPs. The components in the extract were characterized using a Shimadzu FTIR Prestige 21 spectrophotometer (Shimadzu Corporation, Kyoto, Japan) in the KBr phase (1:100).1 (link),2 (link),5 (link) The spectrum was recorded in the range of 400–4000 cm-1. The XRD pattern of the synthesized AME-Ag NPs was constructed using spectral data collected with a Philips PW-1830 X-ray diffractometer. A scanning electron microscope (SEM) (JEOL, Japan) and energy dispersive X-ray spectroscopy system (EDAX Inc.) were used. The AgNPs were observed by transmission electron microscopy (TEM) (JEOL, Japan).
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4

Characterization of Lipid Nanoparticles

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Transmission electron microscopy (TEM) of the LNPs was conducted with an energy-filtered EFTEM Libra 120-Carl Zeiss instrument, Selangor, Malaysia. Particle size and zeta potential measurements of LNPs were performed using dynamic light scattering (DLS) on a Malvern Zetasizer Nano ZS Ver. 7.11 (Malvern Instruments, Malvern, UK). Samples were irradiated with red light from HeNe laser, fitted with a wavelength λ = 633 nm. Besides, chemical functional groups of LNPs were observed via a spectrum FT-IR Prestige-21 spectrophotometer (Shimadzu, Chiyoda-ku, Tokyo, Japan).
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5

Comprehensive Polymer Characterization

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Characterization of the polymers using FT-IR, 1 H-NMR, GPC and LCST was achieved by methods previously reported by our group [35] . FT-IR spectra were recorded with an FT-IR Prestige-21 spectrophotometer (Shimadzu, Japan) in the 4000-500 cm -1 range. 1 H-NMR spectra were recorded by dissolving purified samples (3-5 mg) in d6-DMSO (1 mL) using a Bruker DRX 400 MHz spectrometer (Bruker, Rheinstetten, Germany). The molecular weights (Mw and Mn) and molecular weight distributions of the polymers were determined by GPC measurements on a Waters LS system and molecular weight distributions (Mw/Mn) for the sample polymers were calibrated with standard polystyrene samples. The LCST of the polymers were determined using a UV-vis spectrophotometer (Lambda 35, Perkin Elmer, USA) fitted with a thermostatically controlled cuvette at a heating rate of 0.5 o C/min.
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