The present study used the following three fissure sealants: Teethmate F-1 2.0 (TM; Kuraray Noritake Dental, Tokyo, Japan), a conventional resin-based fissure sealant; BeautiSealant (BS; Shofu, Kyoto, Japan), an S-PRG filler-containing resin-based fissure sealant; and Fuji III LC (IIILC; GC, Tokyo, Japan), an RMGIC-based fissure sealant. Additionally, according to typical methods, the following tooth surface treatment agents were used: K-Etchant Gel (Kuraray Noritake Dental) for TM, an included primer (Shofu) for BS, and cavity conditioner (GC) for IIILC (Table 1).
Beautisealant
Manufactured by Shofu
Sourced in Japan
BeautiSealant is a light-curable sealing agent designed for use in dental laboratories. It provides a protective coating for dental restorations, helping to seal the margins and prevent microleakage.
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5 protocols using beautisealant
1
Comparative Evaluation of Fissure Sealants
2
Sealing Occlusal Fissures with Self-Etch and Glass-Ionomer Sealants
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Self-etch primer (Beautiprimer, Shofu, Kyoto, Japan) was applied to fissures with a brush applicator for 5 s, then dried with a mild air flow according to manufacturer instructions. Giomer-based sealant material (BeautiSealant, Shofu) was applied from the distal to the mesial part of the fissures by using a probe. Ultrasonic vibration was applied to the buccal surface of the specimens for 30 s. Similarly, light curing and storage procedures were performed in the flowable composite groups.
4. Glass-ionomer-based pit and fissure sealant application GC Cavity Conditioner was applied to fissures with a brush applicator for 10 s, then dried with a mild air flow. A glass-ionomer-based sealant material (Fuji Triage, GC, Tokyo, Japan) capsule was activated, triturated for 10 s according to manufacturer instructions, and subsequently loaded to the capsule applier. A thin film of the material was applied into the occlusal fissures and light cured for 30 s. Fuji Coat LC was applied immediately after curing. The specimens were stored in distilled water for 24 h at 37˚C.
4. Glass-ionomer-based pit and fissure sealant application GC Cavity Conditioner was applied to fissures with a brush applicator for 10 s, then dried with a mild air flow. A glass-ionomer-based sealant material (Fuji Triage, GC, Tokyo, Japan) capsule was activated, triturated for 10 s according to manufacturer instructions, and subsequently loaded to the capsule applier. A thin film of the material was applied into the occlusal fissures and light cured for 30 s. Fuji Coat LC was applied immediately after curing. The specimens were stored in distilled water for 24 h at 37˚C.
3
Fluoride-Releasing Sealants Evaluation
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The materials used in this study were a Giomer and three fluoride-releasing sealants divided into 4 groups with eight samples of each sealant: G1 — BeautiSealant (Shofu, Japan); G2 — Clinpro (3M ESPE, USA); G3 — Helioseal F (HF) (Ivoclar Vivadent, Liechtenstein); and G4 — UltraSeal XT (US) plus (Ultradent Products, USA).
The materials were handled according to the manufacturers’ instructions and 32 samples were prepared. The samples consisted of eight blocks of each sealant with 5 mm width and 1 mm thickness; the samples were placed in cavities with similar measures in a Teflon matrix.[7 ]
All samples were polymerized using a LED device (Elipar, 3M ESPE) for 40 s, verifying with a radiometer that the intensity of light emitted has a minimum value of 400 mW/cm2.
The polymerized samples were removed from the matrix and were then stored in plastic bottles with 5 ml of deionized water. The samples were conserved at 37°C for 60 days and measured on days 1, 2, 4, 8, 28, and 60, which is similar to the time intervals used in previous studies.[8 9 (link)]
The materials were handled according to the manufacturers’ instructions and 32 samples were prepared. The samples consisted of eight blocks of each sealant with 5 mm width and 1 mm thickness; the samples were placed in cavities with similar measures in a Teflon matrix.[7 ]
All samples were polymerized using a LED device (Elipar, 3M ESPE) for 40 s, verifying with a radiometer that the intensity of light emitted has a minimum value of 400 mW/cm2.
The polymerized samples were removed from the matrix and were then stored in plastic bottles with 5 ml of deionized water. The samples were conserved at 37°C for 60 days and measured on days 1, 2, 4, 8, 28, and 60, which is similar to the time intervals used in previous studies.[8 9 (link)]
4
Dental Sealant Technologies in Mexican Children
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We conducted a randomized controlled split-mouth clinical trial in elementary school children in Hidalgo, Mexico. Figure 1 shows the methodological design. Our design included the conventional Clinpro sealant (Clinpro©3M, Saint Paul, MN., USA) and one sealant based on Giomer technology (BeautiSealant©Shofu, Kyoto, Japan). Clinpro©3M is a resin-based product that requires an etching adhesive system (12 ). BeautiSealant©Shofu sealant uses Giomer technology with an adhesive system that does not require phosphoric acid (13 ). Different technologies may affect various aspects of performance (e.g., pit penetration (14 (link)), retention (15 (link)) and microfiltration (16 (link)), which was not individually addressed in the present research.
Our research protocol was approved by the Ethics Committee of the Health Sciences Institute at the Autonomous University of the State of Hidalgo (Cinv/o/032/2016).
Our research protocol was approved by the Ethics Committee of the Health Sciences Institute at the Autonomous University of the State of Hidalgo (Cinv/o/032/2016).
5
Evaluation of Fissure Sealant Materials
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The teeth were randomly divided into two preparation groups (invasive treatment and non-invasive treatment) and eight fissure sealant material subgroups (n = 10). The eight fissure sealant materials tested were: a nanofilled flowable composite (Filtek Ultimate Flow, 3M ESPE) a nanohybrid flowable composite (GrandioSo Flow, Voco GmbH, Cuxhaven, Germany), a microhybrid flowable composite (Majesty Flow, Kuraray Noritake Dental, Tokyo, Japan), a resin-based unfilled fissure sealant (ClinPro Sealant, 3M ESPE), a resin-based filled fissure sealant (Fissurit FX, Voco GmbH), a resin-based highly filled fissure sealant (GrandioSeal, Voco GmbH), a giomer-based fissure sealant (BeautiSealant, Shofu Inc., Kyoto, Japan) and a glass ionomer-based fissure sealant (Fuji Triage, GC Corp., Tokyo, Japan) (Table 1).
In the invasive treatment group, enameloplasty was applied to the teeth with a micro narrow taper fissure carbide bur (Fissurotomy Micro NTF, SS White, Lakewood, NJ, USA). The preparation was standardized by extending the fissure entrance to the diameter and half-length of the fissure carbide bur. No bur preparation was performed in the non-invasive group.
In the invasive treatment group, enameloplasty was applied to the teeth with a micro narrow taper fissure carbide bur (Fissurotomy Micro NTF, SS White, Lakewood, NJ, USA). The preparation was standardized by extending the fissure entrance to the diameter and half-length of the fissure carbide bur. No bur preparation was performed in the non-invasive group.
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