Phenotypic analysis was performed on PBMCs using panels containing Live/Dead Blue (Life technologies), CD1c (AD5-8E7; Miltenyi), CD3 (SP34-2 and SK7; BD), CD10 (HI10a; Biolegend), CD11c (B-Ly6, BD), CD14 (M5E2, BD), CD16 (3GE, Biolegend), CD19 (HIB19; Biolegend), CD20 (L27, BD), CD34 (561, Biolegend), CD45RA (HI100, Biolegend), CD56 (HCD56, BD), CD62L (SK11, BD), CD66abce (TET2, Miltenyi Biotec), CD80 (L307.4, BD), CD86 (2331; BD); CD123 (7G3; BD), CD133 (7, Biolegend), CD141 (AD5-14H12; Miltenyi), CD146 (P1H12; Biolegend), HLA-DR (TU36, Life technologies), CCR2 (K036C2, Biolegend), CCR4 (L291H4, Biolegend), CCR6 (11A9, BD) and CCR7 (150503, BD) (Panels summarized in S3 Table ). Fixation was performed with 1% paraformaldehyde. Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences) and analyzed using FlowJo software (Tree Star).
Cd56 hcd56
Manufactured by BD
The CD56 (HCD56) is a cell surface antigen that is expressed on natural killer (NK) cells, a type of lymphocyte involved in the innate immune response. It functions as a neural cell adhesion molecule and plays a role in cell-cell interactions. This product can be used for the identification and enumeration of CD56-positive cells in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Cd66abce tet2, by Miltenyi Biotec (2 mentions)
Cd16 3ge, by BioLegend (2 mentions)
Cd14 m5e2, by BD (2 mentions)
Cd1c ad5 8e7, by Miltenyi Biotec (2 mentions)
Cd11c b ly6, by BD (2 mentions)
Cd19 hib19, by BioLegend (2 mentions)
Cd20 l27, by BD (2 mentions)
Cd123 7g3, by BD (2 mentions)
Cd141 ad5 14h12, by Miltenyi Biotec (2 mentions)
Lsrfortessa flow cytometer, by BD (2 mentions)
Cd45ra hi100, by BioLegend (1 mentions)
Cd3 sp34 2 and sk7, by BD (1 mentions)
Cd80 l307.4, by BD (1 mentions)
Live dead blue, by Thermo Fisher Scientific (1 mentions)
Cd146 p1h12, by BioLegend (1 mentions)
Cd10 hi10a, by BioLegend (1 mentions)
Cd86 2331, by BD (1 mentions)
Cd45 hi30, by BD (1 mentions)
Cd3 sk7, by BD (1 mentions)
Cd83 hb15e, by BioLegend (1 mentions)
2 protocols using cd56 hcd56
1
Comprehensive PBMC Phenotypic Analysis
2
Comprehensive Immune Phenotyping Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Phenotypic analysis was performed on NPA cells and PBMCs using Live/Dead Blue; lineage markers CD3 (SK7; BD), CD19 (HIB19; Biolegend), CD20 (L27, BD), CD45 (HI30; BD), CD56 (HCD56, BD) and CD66abce (TET2, Miltenyi Biotec); HLA-DR (TU36, Life Technologies), CD14 (M5E2, BD), CD16 (3GE, Biolegend), CD11c (B-Ly6, BD), CD1c (AD5-8E7; Miltenyi), CD141 (AD5-14H12; Miltenyi), CD123 (7G3; BD); maturation markers CD83 (HB15e, Biolegend) and CD86 (2331; BD); adhesion marker CD62L (SK11, BD); and migration markers CCR2 (K036C2, Biolegend) and CCR7 (150503: BD); and fixed with 1% paraformaldehyde for flow cytometry. Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences) and analysed using FlowJo software v10 (Tree Star).
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